Effect of long-term frozen plasma on the detection of HBsAg by ELISA
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 1
Abstract
Objective To evaluate the HBsAg detection results of HBsAg+ samples after 8 years refrigeration by ELISA and evaluate the effectiveness of the current storage policy of retained samples. Methods A total of 100 HBsAg+ plasma samples by ELISA from May 2014 to March 2015 and refrigerated at -20℃ were collected and retested for HBsAg using the same method after thawing in 2023. Results The HBsAg retest results of 100 plasma samples were all positive, with the concordance rate of 100%, though there was a significant decrease in the S/CO value after refrigeration(27.52 vs 19.03, P<0.05). Conclusion Long-term refrigeration can lead to a decrease in the S/CO value of HBsAg ELISA detection,but it does not affect the positive results.
Authors and Affiliations
Simin LIAN, Xiaohua CHEN, Shubo LIN, Ziyue GUO, Zebin XIAO
Keywords
Related Articles
- EP ID EP738749
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.01.016
- Views 30
- Downloads 0
Study on relationship between platelet transfusion efficacy and KIR-HLA receptor-ligand compatibility
Objective To study the correlation between platelet transfusion efficacy and KIR receptor-HLA ligand. Methods Thirty-three leukemia patients with positive HLA antibody were tested for cross-matching with donor platelets....
In vitro method establishment for detecting monocyte phagocytosis of sensitized RBCs by flow cytometry
Objective To establish an experimental method for detecting phagocytosis of sensitized red blood cells in vitro by flow cytometry. Methods Mononuclear cells were isolated from the peripheral blood of blood donors and cul...
Establishment and capacity analysis of the platelet donor database with HPA and HLA genotype in Nanjing
Objective To establish a known HPA/HLA genotype platelet donor database in Nanjing, analyze the polymorphism of HPA-1~6w, -15 and HLA-A, -B and evaluate the matching probability and appropriate capacity of the database f...
Platelet antibody of blood donors in Suzhou
Objective To study the incidence and specificity of platelet antibody in blood donors in Suzhou, analyze the distribution characteristics of platelet antibody in blood donors in this area, and explore the significance of...
Identification and clinical transfusion of B (A) subgroup
Objective To carry out serological and molecular biological identification of B (A) subtype, and discuss the rational blood transfusion strategy. Methods Serological and direct sequencing methods were used to detect sero...