Establishment and capacity analysis of the platelet donor database with HPA and HLA genotype in Nanjing
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 10
Abstract
Objective To establish a known HPA/HLA genotype platelet donor database in Nanjing, analyze the polymorphism of HPA-1~6w, -15 and HLA-A, -B and evaluate the matching probability and appropriate capacity of the database for platelet matching transfusion. Methods HPA-1~6w, -15 and HLA-A, -B were genotyped by PCR-SSP and PCR-SBT, respectively. The allele frequency and haplotype frequency of HPA-1~6w, -15 and HLA-A,- B were calculated with SHEsis software, and then the matching probability and appropriate capacity were obtained according to the haplotype frequencies. Results The population genetic polymorphisms data of HPA-1~6w, -15 and HLA-A, -B in Nanjing were obtained. According to the subsequent calculating, without considering ABO blood type, in a database size of 527 donors, a patient with haplotype frequency >0.001 has approximate 95% probability to achieve matching of HPA-1~6w, -15 genotype. A database with a total size of 1 875 donors can afford the patient with haplotype frequency >0.001 to find at least 1 HLA-A, -B matched donor in 95% probability. Conclusion We established a local platelet donor database with known HPA and HLA genotype, which also provided important data support for the subsequent construction, maintenance and clinical application of the database.
Authors and Affiliations
Taixiang LIU, Ling MA, Wenbiao LIANG, Nizhen JIANG, Ling LI
Keywords
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- EP ID EP749835
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.10.006
- Views 37
- Downloads 0
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