DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 2
Abstract
Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model. Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats. Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively. Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.
Authors and Affiliations
RAM NARENDRAN R, MALEEKA BEGUM SF, RUBAVATHI S
Keywords
Related Articles
- EP ID EP608760
- DOI -
- Views 106
- Downloads 0
MOLECULAR DOCKING STUDIES AND BIOACTIVITY OF VARIOUS BENZILIC ACIDS AND ITS ANALOGS
Objective: Various benzilic acids and its analogs have been synthesized using the protocol, obtain good to exceptional yield and their biological activity, and its docking studies have been discussed.Methods: Molecular d...
SMARTPHONE ADDICTION AND ITS RELATIONSHIP WITH PSYCHOLOGICAL HEALTH AMONG STUDENTS OF A MEDICAL SCHOOL IN EAST COAST MALAYSIA
Objective: Smartphones have now become a common and almost essential tool in our daily living activities. Students are among those who use smartphones regularly, which could lead to addiction if overused. This study aims...
PACKAGING OF HEPATITIS B SURFACE ANTIGEN VACCINE AND MORINGA OLEIFERA EXTRACT INTO CHITOSAN NANOPARTICLES
Objective: Oftentimes, the recombinant antigen for the use of vaccines is less immunogenic than live attenuated or inactive vaccines. Hence, a potent adjuvant is needed to enhance the immune response. Moreover, the role...
MOLECULAR DOCKING STUDIES ON EGF – 5 FLUOROURACIL
Cancer can be described as the uncontrolled growth of abnormal cells. 5 Fluorouracil is an anticancer drug which has it effects on colon cancer, brain tumor, breast cancer, head & neck cancer. The aim of the present...
PROTECTIVE OF ETHANOLIC EXTRACT OF SAUSSUREA LAPPA AGAINST PARACETAMOL-INDUCED HEPATIC AND RENAL DAMAGE IN MALE RABBITS
Objective: The objective of our study is to investigate the effect of the ethanolic extract of Saussurea lappa against paracetamol-induced hepatorenal toxicity in male rabbits. Methods: Eighteen male rabbits were used f...