Development And Characterization of Inhaled Chitosan Nanoparticles Loaded with Isoniazid
Journal Title: JOURNAL OF PHARMACEUTICAL TECHNOLOGY, RESEARCH AND MANAGEMENT - Year 2014, Vol 2, Issue 2
Abstract
The objective of our study is to load first line anti-tubercular drug, Isoniazid in chitosan Nanoparticles in order to enhance bioavailability and to reduce dose frequency. The chitosan nanoparticles containing the drug Isoniazid were prepared by the method of spontaneous emulsification. Chitosan gel containing drug is cross linked with Glutaraldehyde and nanoparticle suspension obtained was centrifuged at 5000 rpm. It was then evaluated for Drug loading, swelling index, Mucoadhesive force, Zeta potential, DLS studies, DSC studies, SEM studies, In vitro Drug release, Pharmacokinetic Studies and Stability studies.Formulation 1(F1) shows maximum Drug Loading, Swelling index and mucoadhesive force. The positive zeta value was obtained for all formulations due to positive charge of polymer used in preparation of dispersion. The DLS plot of Formulations shows that Average particle diameter are in the range of 661.8-823.8nm. The SEM study revealed that the micrographs of cross linked chitosan nanoparticles have smooth surface. The thermogram of the formulations showed the shifting of endotherm. This indicates the possible change in the release kinetics and bioavailability of the drug. In vitro drug releases was found to be maximum for formulation F6. Pharmacokinetic evaluation shows all the formulation shows first order rate release profile and release mechanism from nanoparticles is diffusion controlled. Stability studies indicated that the developed chitosan nanoparticles are physically and chemically stable and retain their pharmaceutical properties at various environmental conditions over a period of 3 months.
Authors and Affiliations
Junise V , R Saraswathi
Keywords
Related Articles
- EP ID EP596691
- DOI 10.15415/jptrm.2014.22011
- Views 109
- Downloads 0
Toxicological Evaluation of Crude Alkaloid Fraction Isolated From Indian Folklore Plant Telosma Pallida (Roxb) wg Craib Root Using Probit Value Analysis
Present study first time reported the toxicological profile of Telosma pallida (TP) crude alkaloid fraction (CAF) isolated from the root of the climber plant by brine shrimp lethality test (BLT). Telosma pallida is a per...
Cold Chain: A Lynchpin of National Immunization Program
Cold chain management (CCM), a lynchpin of National Immunization Program (NIP) is to deliver the vaccines or other immunological products/ vaccines to right people, at right place, and in right time ineffective m...
A Study of Prescribing Habits of Low Molecular Weight Heparin in Cardiovascular Disease in Tertiary Care Hospital
Background and Objective: Cardiovascular Diseases (CVDs) are the leading non-communicable diseases and also leading cause of morbidity and mortality worldwide. Low Molecular Weight Heparin (LMWHs) is frequen...
Pulsatile Drug Delivery System – A Novel Approach for Time and Spatial Controlled Drug Delivery
The area of pharmaceutical research is broadened with the invention of new pharmaceutical drug delivery system. The traditional drug deliveries systems have not the way to treat or cure special disease or as well as the...
Ellagic Acid Administration Reverses Colchicine- Induced Dementia in Rats
The late-onset sporadic type of Alzheimer’s disease is characterised by chronic oxidative stress, neuroinflammation and cognitive dysfunction. Ellagic acid is a naturally occurring polyphenol known to possess robust anti...