Development and validation of a HPLC method for the determination of amoxicillin trihydrate, colistin sulphate, nipasol and nipagin in an injectable suspension
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2016, Vol 10, Issue 1
Abstract
A liquid chromatographic method has been developed and validated for simultaneous determination of amoxicillin trihydrate, colistin sulphate, nipasol and nipagin in injectable suspension. Efficient chromatographic separation was achived on a Hypersil Gold (150mm x 4.6mm, 5.0 µm) with mobile phase containing 4.46 g‰, pH 2.5 (adjusted with dilute sulphuric acid) in gradient with acetonitrile at a flow rate of 1.0 mL/min. detection of the analyses was performed at different wavelengths using a DAD detector. The elution was a seven step gradient elution program in 42 minutes. The proposed HPLC method was statistically validated with respect to specificity, linearity, limits of detection and quantification, ranges, precision and accuracy. The HPLC method was applied to injectable suspension in which the analyses were successfully quantified with no interfering peaks from excipients
Authors and Affiliations
Violeta Tauber, Viorica Chiurciu
Keywords
Related Articles
- EP ID EP117956
- DOI -
- Views 116
- Downloads 0
Report regarding the microbiological quality control of the veterinary pharmaceutical products during 2013 (In Romanian)
The microbiological contamination of pharmaceutical products could have major consequences on the physical-chemical characteristics, safety and efficiency of the medicinal products and also on the out of specification pr...
Guide of usual calculations for veterinary practician
In his current activity in the prescription and administration of medicines the vet is obliged to apply often to usual calculations in veterinary medicine. By those calculations’ correctness it depend the success of ther...
Therapeutic and lesional aspects of feline infectious peritonitis
The prevalence of feline infectious peritonitis (FIP) can not be assessed on the basis of serological surveys because positive serological reagents rate does not correlate with disease rates. In units with more cats and...
Report concerning the colaborative study for establish of reference standard eficacity of Teicoplanin
The Microbiology Laboratory from the Institute for the Control of Veterinary Biological Products and Medicines participated to a collaborative study in order to determine the potency of teicoplanin CRS, batch 2, accordin...
Procesul de dezinfectare al aerului și suprafețelor prin formarea unei cei foarte fine de 5 μm, fără umezire, pe baza de peroxid de hydrogen – nocolyse generată de nebulizatorul Nocospray
Nocospray este un concept revolutionar destinat bio dezinfectiei profesionale, cu actiune bactericidă, virucidă, fungicidă si sporicidă, utilizând ca substantă activă peroxidul de hidrogen în combinaie cu argint colo...