Development and validation of a RP- HPLC method for the quantittation studies of metronidazole and furazolidone from product Enteroguard M
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2010, Vol 4, Issue 2
Abstract
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250 mm x 4,6 mm i.d.; 5 +m particle size), held at 25°C. The mobile phase consisted of methanol/ 0,1% phosphoric acid aq.(20/ 80 v/ v), run at flow rate of 1 mL/min and with UV detection at 317 nm. Method validation investigated parameters such as linearity (r2 = 0. 9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of ENTEROGUARD M tablets.
Authors and Affiliations
Elena Oltean, B. Milea
Keywords
Related Articles
- EP ID EP97148
- DOI -
- Views 128
- Downloads 0
The effect of treatment with Amprolium and Sulfadiarom in an episode of ovine coccidiosis in Timis County
Coccidiosis is an intestinal parasitic disease that affects sheep and goat young people under one year of age, manifested by diarrhea often accompanied by bleeding or constipation and weakness, being produced by Eimeria...
Development and validation of a RP- HPLC method for the quantittation studies of metronidazole and furazolidone from product Enteroguard M
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out...
Interactiunea de tip sinergic dintre paracetamol (acetaminofen) si carbamazepină la soarece
Eficacitatea pastei antihelmintice FENBENDAZOL 30% în ciatostominoza cabalinelor
Ciatostominoza cabalinelor este o helmintoză a intestinului gros produsă de paraziți din familia Strongylidae, subfamilia Cyathostominae. Ciatostominele (strongili nemigratori) reprezintă o provocare atât pentru paraz...
A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances
The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substanc...