Development and validation of a RP–HPLC method for the quantization studies of metronidazole in tablets and powders dosage forms
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2011, Vol 5, Issue 2
Abstract
An isocratic high–performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole in tablets and powders dosage forms. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250 mm x 4.6 mm i.e.; 5 µm particle size), held at 25°C. The mobile phase consisted of methanol/ 0.1% phosphoric acid aq. (20/ 80 v/v), run at flow rate of 1 mL/ min and with UV detection at 317 nm. Method validation investigated parameters such as linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of tablets and powders dosage forms.
Authors and Affiliations
Elena Oltean
Keywords
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