Development and Validation of a Simple, Sensitive, Selective and Stability-Indicating RP-HPLC Method for the Determination of Darunavir and Cobicistat in pharmaceutical combined dosage forms
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
The study describes development and subsequent validation of a stability indicating reverse-phase HPLC method for simultaneous estimation of the Darunavir and Cobicistat in combined Pharmaceutical dosage form using RP-HPLC. Separation was accomplished on BDS 250 x 4.6 mm, 5m C18 column using 0.1% Perchloric acid buffer and acetonitrile (38:62 v/v) as mobile phase pumped through at a flow rate of 1 ml/min at 30°C. Optimized wavelength was 211 nm, retention time of Darunavir and Cobicistat were found to be 2.24 min and 2.63 min respectively. % RSD of the Darunavir and Cobicistat were found to be 0.3 and 0.5 respectively. Mean recovery were found to be 100.0% and 100.04% for Darunavir and Cobicistat respectively. The proposed method also proved to be suitable as a rapid and reliable quality control method.
Authors and Affiliations
Mathews Bommella1, R. N. Rao1 ,, Mukkanti Khagga1, Sarbani Pal2
Keywords
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- EP ID EP390004
- DOI 10.9790/3008-1204036978
- Views 88
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