DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RP-HPLC METHOD FOR THE DETERMINATION OF VALSARTAN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 8
Abstract
Objective: A stability indicating RP-HPLC method was developed and validated for the determination of Valsartan using Telmisartan (10 µg/ml) as the internal standard.Methods: In this procedure Phenomenex ODS C-18(250×4.6 mm, packed with 5 micron) column was used with a new mobile phase consisting of methanol: acetonitrile: water (70:15:15 v/v) and the pH was adjusted to 3 by 0.1% glacial acetic acid with a flow rate of 1 ml/min. The eluents were monitored at 249 nm. Valsartan was subjected to stress conditions including hydrolytic degradation in acidic, basic and neutral conditions, oxidation, photolytic, UV degradation and thermal degradation.Results: Linearity was obtained in the concentration range of 10-90 µg/ml (R2 =0.999) and with a regression equation y=0.074x+0.005. The LOD and LOQ values were 0.261 and 0.791 µg/ml respectively. The drug had shown promising degradation in the acidic, basic, neutral, thermal and oxidative stress conditions.Conclusion: The method was validated for accuracy, precision, linearity, specificity and robustness and revealed that it is specific, accurate, rapid, precise, reliable and reproducible enough to analyze commercial dosage forms as per ICH guidelines.
Authors and Affiliations
K. K. Pradhan, U. S. Mishra
Keywords
Related Articles
- EP ID EP578240
- DOI -
- Views 88
- Downloads 0
A PROSPECTIVE STUDY: KNOWLEDGE ASSESSMENT AND PATIENT CARE OF DIABETIC FOOT ULCER PATIENTS IN TERTIARY CARE HOSPITAL
Objective: To assess the knowledge with a diabetic foot ulcer, to evaluate the severity and grade of diabetic foot ulcer, to study the self-care behaviour and medication adherence in a patient with diabetic foot ulcer an...
INVESTIGATION OF THE ANTIBIOTIC RESISTANCE: THE CASE OF BUU DIEN GENERAL HOSPITAL IN HO CHI MINH CITY
Objective: In Vietnam, antibiotic resistance has been gained the attention of medical professionals in antibiotic use management. This study aimed to investigate the antibiotic resistance among hospital-acquired infectio...
SOLID DISPERSION TECHNIQUE TO ENHANCE THE SOLUBILITY AND DISSOLUTION RATE OF ARIPIPRAZOLE BY FUSION METHOD
Objective: The objective of the present study was to enhance the solubility and dissolution rate of Aripiprazole (APZ), a water insoluble drug, by a solid dispersion technique.Methods: Solid dispersions (SD) of APZ were...
EFFECTS OF ETHANOL ON MOUSE EMBRYONIC STEM CELL DIFFERENTIATION
Objective: Chronic alcohol consumption during gestation causes fetal malformations, termed fetal alcohol syndrome (FAS). We conducted the present study to clarify the mechanism underlying alcohol consumption-induced malf...
LAWSONIA ALBA LEAVES INDUCE APOPTOSIS AND CELL CYCLE ARREST IN B16F10 MELANOMA CELLS
Objective: The present study was designed to investigate the anti-melanoma activity of the ethyl acetate fraction of Lawsonia alba lam leaves (ELA) against B16F10 cells.Methods: Cytotoxicity of ELA on B16F10 cells was de...