DEVELOPMENT AND VALIDATION OF ABSORBANCE CORRECTION METHOD FOR SIMULTANEOUS DETERMINATION OF LEVOCETIRIZINE AND AMBROXOL IN COMBINED TABLET DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2015, Vol 6, Issue 3
Abstract
A new, simple, accurate and sensitive UV-Spectrophotometric absorbance correction method has been developed and validated for simultaneous estimation of Levocetirizine (LEV) and Ambroxol (AMB) in a combined tablet dosage form. Distill water was used as solvent. In this method, two wave lengths on Ambroxol curve were selected where it showed same absorbance, which were 261 nm and 303 nm. At 303 nm, Levocetirizine showed zero absorbance while at 261 nm it gave considerable absorbance. At 261 nm, both the drugs showed some absorbance. The absorbance of Levocetirizine was corrected at 261 nm. The method was found to be linear between the range of 4-28 μg/ml for Levocetirizine and Ambroxol. The mean percentage recovery was found in the range of 99.4 ± 1.11 % and 100.2 ± 1 % for Levocetirizine and Ambroxol respectively at three different levels of standard additions. The precision (intra-day, inter-day) of method were found within limits (RSD < 2 %). The method was validated according to ICH guidelines. Thus the proposed method was simple, precise, economic, rapid and accurate and can be successfully applied for simultaneous determination of Levocetirizine and Ambroxol in combined tablet dosage form.
Authors and Affiliations
Nancy Thakkar, Satish Patel
Keywords
Related Articles
- EP ID EP163613
- DOI 10.7897/2230-8407.06349
- Views 96
- Downloads 0
VAJIKARANA: A UNIQUE THERAPY OF AYURVEDA
Ayurved, the science of life deals with every aspect of life and Vajikarana is one of its eight branches being practiced since time immemorial. Ayurveda has two aims i.e. protection and promotion of health and secondly c...
CRIMEAN CONGO HEMORRHAGIC FEVER - AN ARTICLE REVIEW
Crimean–Congo hemorrhagic fever (CCHF) caused by negative-sense, single-stranded RNA virus in the genus Nairovirus, family Bunyaviridae. (CCHF) is a tick-borne infectious disease characterized by fever, malaise, headache...
ANTIMICROBIAL POTENTIAL OF VOLATILE OIL ISOLATED FROM SOME TRADITIONAL INDIAN SPICES
In the present study the antimicrobial activity of Volatile Oils isolated from Traditional Indian Spices, Anethum Graveolens (Umbelliferae), Foeniculum Vulgare (Umbelliferae) and Coriandrum Sativum (Umbelliferae) were s...
COMPARATIVE STUDY OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ULTRAVIOLET SPECTROPHOTOMETRY METHODS ON DIFFERENT BRANDS OF METRONIDAZOLE TABLETS MARKETED IN MAIDUGURI METROPOLITAN COUNCIL OF BORNO STATE
The active ingredient of ten brands of Metronidazole tablets were determined using High Performance Liquid Chromatography and Ultra Violet Spectrophotometry to determine which of the assay methods is better and if the dr...
AN OVERVIEW: THE ROLE OF PROCESS VALIDATION IN PHARMACEUTICAL INDUSTRY
The present review article focus on introduction and general overview on process validation in pharmaceutical industry. The word validation simply means “Assessment of validation or action of proving effectiveness”. The...