Development and Validation of Dual Wavelength UV Spectrophotometric Method for simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in Tablet dosage form
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2014, Vol 2, Issue 1
Abstract
A simple and economical dual wavelength spectrophotometric method has been developed for the simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in their tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the overlain spectra is directly proportional to the concentration of the component of interestâ€. From the UV absorption spectrum of Cilnidipine, three wavelengths were selected, which were 282.99, 337.85 and 352.92 nm. At 352.92 nm only Cilnidipine has reasonable absorbance, so it was selected for the estimation of it from combination drug product. At these two wavelengths absorbance for Cilnidipine was found to be same i.e. absorbance difference was zero for any concentration, while for Olmesartan Medoxomil concomitantly increase in absorbance difference with increase in its concentration. So, 282.99 and 337.85 nm wavelengths were selected for the estimation of Olmesartan Medoxomil from its combination drug product. The method involved solving of an equation based on measurement of absorbances at two wavelengths 282.99 and 337.85 nm. Regression analysis of Beer’s plots showed good correlation in concentration range of 10-60 μg/ml for Cilnidipine and 20-120 μg/ml for Olmesartan Medoxomil. The suitability of this method was for quantitative determination of Cilnidipine and Olmesartan Medoxomil was proved by validation and recovery study. The proposed method was found to be simple, rapid, economical, accurate and reproducible for the routine analysis of both drugs in tablet dosage forms.
Authors and Affiliations
Isha J. Soni| Quality assurance department, Arihant School of Pharmacy & Bio Research institute, Uvarsad cross road, Adalaj, Gandhinagar, Gujarat, India, Hiral J. Panchal| Quality assurance department, Arihant School of Pharmacy & Bio Research institute, Uvarsad cross road, Adalaj, Gandhinagar, Gujarat, India
Keywords
Related Articles
- EP ID EP14105
- DOI -
- Views 349
- Downloads 18
Role of arbuscular mycorrhiza fungi on tolerance to salinity of the tree legume Albizia lebbeck (L.) inoculated by Rhizobium
Myrica esculenta (Myricaceae) and Syzygium cumini(Myrtaceae), the Indian traditional fruits The effect of different level of salinity on growth, nodulation and Nitrogen fixation of single and dual inoculated tree legum...
Morphometric Approach towards Growth Performance of Mahseer (Tor tor) in River Narmada Near Hoshangabad (M.P)
During present study total 58 specimens of Mahseer (Tor tor) from River Narmada were studied for morphometric and meristic characteristics. Interrelationship among the morphometric and meristic characteristics were obs...
Antimicrobial Screening of Some capparidaceous Medicinal Plants of North-West Rajasthan
Antimicrobial screening of ethyl ether and alcoholic extracts of leaves of three selected medicinal plant species of capparidaceae family growing in North–Western Rajasthan was carried out. Capparis decidua, Cleome g...
Capparidaceous Medicinal Plants of North-West Rajasthan: Good Sources of Ascorbic Acid
Ascorbic acid contents of roots, shoots and fruits of three selected medicinal plants like Capparis decidua, Cleome gynandra and Cleome viscosa of family Capparidaceae have been analysed. Maximum ascorbic acid contents...
Assessment of visual problems in elderly in an urban slum community of Mumbai
Background: Visual problems in the elderly share a major portion of the disability in the elderly. Visual impairment in the elderly can interfere with daily activities tremendously leading to dependence in many things....