Development and Validation of Dual Wavelength UV Spectrophotometric Method for simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in Tablet dosage form
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2014, Vol 2, Issue 1
Abstract
A simple and economical dual wavelength spectrophotometric method has been developed for the simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in their tablet dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the overlain spectra is directly proportional to the concentration of the component of interestâ€. From the UV absorption spectrum of Cilnidipine, three wavelengths were selected, which were 282.99, 337.85 and 352.92 nm. At 352.92 nm only Cilnidipine has reasonable absorbance, so it was selected for the estimation of it from combination drug product. At these two wavelengths absorbance for Cilnidipine was found to be same i.e. absorbance difference was zero for any concentration, while for Olmesartan Medoxomil concomitantly increase in absorbance difference with increase in its concentration. So, 282.99 and 337.85 nm wavelengths were selected for the estimation of Olmesartan Medoxomil from its combination drug product. The method involved solving of an equation based on measurement of absorbances at two wavelengths 282.99 and 337.85 nm. Regression analysis of Beer’s plots showed good correlation in concentration range of 10-60 μg/ml for Cilnidipine and 20-120 μg/ml for Olmesartan Medoxomil. The suitability of this method was for quantitative determination of Cilnidipine and Olmesartan Medoxomil was proved by validation and recovery study. The proposed method was found to be simple, rapid, economical, accurate and reproducible for the routine analysis of both drugs in tablet dosage forms.
Authors and Affiliations
Isha J. Soni| Quality assurance department, Arihant School of Pharmacy & Bio Research institute, Uvarsad cross road, Adalaj, Gandhinagar, Gujarat, India, Hiral J. Panchal| Quality assurance department, Arihant School of Pharmacy & Bio Research institute, Uvarsad cross road, Adalaj, Gandhinagar, Gujarat, India
Keywords
Related Articles
- EP ID EP14105
- DOI -
- Views 328
- Downloads 18
Ulcerative colitis in 17 yr old girl masquerading as severe anemia: A case report
Ulcerative colitis is a chronic inflammatory disease of unknown etiology. It is localized to the colon and spares the upper gastrointestinal tract. Ulcerative colitis can occur at any age, the peak incidence is among t...
Beekeeping and Bee Products; Boon for Human Health and Wealth
The cross-fertilization by bee increases average agricultural yield by 20 to 25 percent. Its products like honey, pollen, royal jelly, propolis, bee venom have immense medical importance. Honey is useful for healing the...
A review on gastroretentive drug delivery system
Oral controlled release and site specific drug delivery system has been of great interest in pharmaceutical field to achieve improved therapeutic advantage. Concept of novel drug delivery system arose to overcome certain...
Antimicrobial activity and Characterization of Marine bacteria
Marine bacteria were isolated from seawater was collected from different coastal areas of the Tamilnadu Sea. The antimicrobial activities of these bacteria were investigated. Ethyl acetate extracts of marine bacterial...
Flavonoid Contents From Some Capparidaceous Medicinal Plants of North-West Rajasthan
Evaluation of flavonoid contents from three selected medicinal plant species of capparidaceae family growing in North–Western Rajasthan was carried out. The leaves of Capparis decidua, Cleome gynandra and Cleome visc...