Development and validation of HPLC method for the estimation of Escitalopram oxalate in tablets.
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2013, Vol 2, Issue 1
Abstract
A simple, specific, robust, accurate and precise isocratic HPLC method has been developed and subsequently validated for simultaneous determination of escitalopram (ESP) in pharmaceutical dosage forms. Kromosil (250x4.6)mm 5ยต with flow rate of 1ml/ min by using JASCO PU-1580 and UV/VIS JASCO UV-1570 at 238 nm. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 5mM ammonium acetate buffer (pH 3.0) in the ratio 30:20:50 respectively. The retention time for escitaloparm was found to be 5.36 minutes respectively. The correlation coefficient was found to be 0.9997 (ESP). The mean percentage recovery was found to be 101.86 respectively. The % estimation of the drugs was found near to 100 % representing the accuracy in the method. The proposed method was also validated and applied for the analysis of drugs in tablet formulation.
Authors and Affiliations
Ramathilagam N
Keywords
Related Articles
- EP ID EP648973
- DOI -
- Views 80
- Downloads 0
Analytical method development and validation for residual solvent of Diltiazem hydrochloride extended release capsule by Gas chromatography
Residual solvents are organic volatile chemicals that are either used or produced during the manufacture of active pharmaceutical ingredients, excipients, and drug products. Organic solvents such as di ethyl ether, N hep...
Antibacterial activity on leaf extracts of Syzgium jambalonam
The purpose of this investigation was to extract the bioactive agents from the Methanol, Acetone extracts were examined for their activities against pathogenic microorganism (Proteus vulgaris, Staphyloccus aureus, Bacill...
RP-HPLC Method Development and Validation for the Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Tablet Dosage Form
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of ATENOLOL and INDAPAMIDE in pharmaceutical tablet dosage form. Chromatogr...
Synthesis, characterization of certain new heterocyclic hybrids of pyrazoles and evaluation of their anti-inflammatory activity
The work presented in this article consists of synthesis, characterization and biological evaluation of substituted pyrazole derivatives. Pyrazole derivatives have been shown to have wide variety of pharmacological activ...
Method development and validation for the simultaneous estimation of sitagliptin and metformin in tablet dosage form by RP-HPLC
A RP-HPLC method was developed and validated for the simultaneous estimation of Metformin Hydrochloride (MET) and Sitagliptin (STG) in pure and pharmaceutical dosage form. Chromatography was carried on Phenomex (kromosil...