Development and validation of HPTLC method for determination of Telmisartan in API and pharmaceutical dosage form
Journal Title: World Journal of Pharmaceutical Sciences - Year 2017, Vol 5, Issue 5
Abstract
The aim of present work was to develop a simple and sensitive, HPTLC for the quantitative estimation of Telmisartan in its single component tablet formulations (40 mg). Telmisartan was chromatographed on silica Gel 60 F254 TLC plate using Toluene:Methanol (7:3 v/v/v) as mobile phase. Telmisartan in methanol scanned by Camag TLC scanner 4 with UV visible detector over wavelength range 200 to 400 nm, showed Rf value of 0.46 at wavelength of 299 nm and selected for further studies. The method was validated in terms of linearity (1-3 ng/ml), precision (intra-day variation 1.61, inter-day variation 2.73), accuracy (81.55 to 87.51%) and specificity. The limit of detection and limit of quantification for Telmisartan were found to be 0.25 ng/spot and 0.7 ng/spot, respectively. It can be concluded from the results that the proposed method was accurate, precise and consistent the determination of Telmisartan in Tablet dosage form. This method was validated as per ICH guideline Q2 (R1). Results suggest that this method can be used for routine estimation of Telmisartan in bulk and pharmaceutical dosage forms.
Authors and Affiliations
Manisha S. Phoujdar
Keywords
Related Articles
- EP ID EP330249
- DOI -
- Views 65
- Downloads 0
Detection of human cytomegalovirus pp65 after gene fragmentation by nested PCR
The pp65 capable of modulating the rapid induction of an interferon-like response in cells that follows virus binding and penetration. Two hindered blood samples were obtained from selected pregnant women with history of...
An introduction to Diabetic Neuropathy: A Review
Diabetic neuropathy is the most frequent and devastating complication of diabetes mellitus leading to great morbidity and mortality, resulting in a massive financial burden for diabetes care. The prevalence of diabetic n...
Chemical analysis, antioxidant and antimicrobial activities of leaves essential oil of Annona senegalensis pers. from Burkina Faso
The aim of this study was to determine the chemical composition, antioxidant and antimicrobial properties of leaves essential oil of Annona senegalensis from Burkina Faso. Essential oil was obtained by hydrodistillation...
Pharmacoeconomic evaluation of domestic original preparation "succinasol"
A new domestic preparation “Succinasol” is presented in this paper. According to its studied description it is the most appropriate agent for the purposes of detoxification, salt regulation, acid alkaline balance correct...
Actomyosin filaments and ATP - heart of rigor mortis
It is a well-known fact that contractile substance of the muscle fibril is actomyosin, a complex composed of two protein filaments- actin (thin filament) and myosin (Thick filament), which require adenosine triphosphate...