Development and Validation of new RP-HPLC Method for Simultaneous Estimation of Drug Bupropion and Zonisamide in Tablet Dosage Form
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 5
Abstract
A simple, economic, accurate and precise reverse phase high performance liquid chromatography method for analysis of Bupropion and Zonisamide was developed and validated according to ICH guidelines. The quantification of the drug was carried out using PDA (photodiode array) detector. Column in isocratic mode, with mobile phase Methanol : Buffer in ratio of 80:20 was used. The flow rate was 1.0 ml/min and effluent was monitored at 252nm.the retention times were 2.841 and 3.258min for Bupropion and Zonisamide respectively. The injection volume was 20µl.as per ICH guidelines the method was validated and the method was found to be linear in the range of 20-80µg/ml for Bupropion and Zonisamide. Percent recovery studies of Bupropion and Zonisamide 99.77%and100.29%. The limit of detection and quantification was found to be 0.34&1.05µg/ml Bupropion and 0.25&0.77µg/ml for Zonisamide .the values of precession and robustness lie within the acceptance limit. Thus the proposed method can be successfully applied for simultaneous determination of Bupropion and Zonisamide in routine analysis work.
Authors and Affiliations
Paleti Mahalakshmi
Keywords
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