Development and Validation of New RP-HPLC Method for the Determination of Zolpidem Tartrate in Pure and Pharmaceutical Formulations
Journal Title: Scholars Academic Journal of Pharmacy - Year 2013, Vol 2, Issue 5
Abstract
The present work is concerned with application of simple, precise, accurate, reproducible and specific RP-HPLC method for estimation of Zolpidem Tartrate (ZPT) in bulk and pharmaceutical formulations. Separation of ZPT was successfully achieved on a Symmetry XTerra C18 (4.6 x 150mm, 5 m) Waters or equivalent in an isocratic mode utilizing Ammonium Acetate Buffer (4.5 pH): Methanol (40:60%v/v) at a flow rate of 0.8 mL/min and eluate was monitored at 300 nm, with a retention time of 3.14 minutes. The method was validated and the response was found to be linear in the drug concentration range of 20 µg/mL to 60 µg/mL. The values of the slope, intercept and the correlation coefficient were found to be 47336, 13206 and 0.999 respectively. The RSD values for system precision and method precision were found to be 0.14 % (Intra-day), 0.18% (Inter-day) and 0.46 % (Intra-day), 0.14 % (Inter-day) respectively. Accuracy of method was determined through recovery studies which were found to be 98.90-100.87%. Keywords: Zolpidem Tartrate(ZPT), RP-HPLC, Acetate Buffer (4.5 pH), Methanol.
Authors and Affiliations
M. Kiran, S. Ushasri, Srinadth Nissankarao
Keywords
Related Articles
- EP ID EP378371
- DOI -
- Views 85
- Downloads 0
Toxicity evaluation of Abrusprecatorius seeds collected from Bunda District, Tanzania
Abstract:The plant Abrusprecatorius is found in tropical countries and widely used as an abortifacient, oral contraceptive and anti-nausea. Seeds are the most poisonous part of the plant due to a toxic poison - abrin. In...
Gender Analysis of the Strategy against AIDS in the Active Military in Saint-Louis (Senegal)
Since 2005, Senegal has a national strategy document for equality and gender equity with two objectives of establishment of an institutional, legal and socio-cultural economic environment conducive to the achievement of...
Evaluation of the Leaves of the Gynocardia odorata Plant for Antibacterial Activity
The present investigation was undertaken to appraise the antibacterial activity of the leaves of the Gynocardia odorata plant. The study covered the various macroscopic and microscopic behaviors of the proposed leaves. T...
Chemopreventive Influence of Kolaviron on 1,2-Dimethylhydrazine Induced Plasma Carbonyl Content and Non-Enzymatic Antioxidant Status in Rat Colon Carcinogenesis
Colon cancer is the second leading cause of cancer death worldwide with diet playing a prominent role in disease initiation and progression. We have investigated the chemopreventive efficacy of kolaviron on plasma protei...
A New Method Development and Validation for Quantitative Estimation of Olmesartan Medoxomil in Pharmaceutical Dosage form by UV
A Simple, Sensitive,Precise,Accurate and Linear Spectroscopic Method has been Developed and Validated for the Estimation of Olmesartan Medoxomil in Tablet Dosage form. The λmax of Olmesartan Medoxomil was Determined by u...