DEVELOPMENT AND VALIDATION OF Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RIFAMPICIN AND ITS BIOENHANCER; 3’, 5-DIHYDROXYFLAVONE-7-O-Β-D-GALACTURONIDE-4’-O-Β-D-GLUCOPYRANOSIDE; IN BULK AND FORMULATION
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2019, Vol 8, Issue 5
Abstract
The present research work demonstrates an analytical method development for simultaneous estimation of Rifampicin (RIF) and its bioenhancer; 3’,5-dihydroxyflavone-7-O-β-D-galacturonide-4’-O-β-D-glucopyranoside (CC-I) in combined dosage form using Q-absorbance ratio concept. While method development, two different wavelengths one representingIso-absorptive point (370 nm) and other representing the λmax of Rifampicin (239 nm) were used. Optimum response was obtained in solvent system that comprisesmethanol and water in ratio of 80:20 v/v.Proposed UV method was found to be linear over the concentration range of 2-20 µg/ml for Rifampicin and that of 1-24 µg/ml for CC-I.On the basis of recovery studies after standard addition, accuracy of proposed method was found to be in between 99.94 to 100.30 and 99.90 to 99.96 % for RIF and CC-I respectively. Intra-day precision of the method in terms of % relative standard deviation was found to be in between 0.21 to 1.36 and 0.21 to 1.77 for RIF and CC-I respectively. Inter-day precision range of the method for RIF and CC-I was found to be in between 0.13 to 1.94 and 0.11 to 1.58 respectively.LODand LOQ of proposed UV method were 0.043 and 0.014 µg/ml for RIF and 0.37 and 0.12 µg/ml for CC-I.Proposed UV method was robust and rugged in nature.Proposed methodwas successfully used for the estimation of RIF and CC-I contents of in-house formulation consisting of APIs and the common excipients.
Authors and Affiliations
Sachin Shivling Bhusari, Shreya Hiralal Waghmare, Pravin Shridhar Wakte
ROLE OF NANOBIOTECHNOLOGY IN PHARMACY AND MEDICINE: A REVIEW
Nanobiotechnology is a term, which describes the combination of the two different worlds of engineering and molecular biology. It is a fusion of the following words: “nano” meaning very small, “bio” meaning living, and “...
TECHNICAL SHEET OF FRESH BARK OF THE “WHITE VARIETY” OF BYTTNERIA CATALPIFOLIA, A WILD PLANT CONSUMED IN THE WESTERN PART OF CÔTE D’IVOIRE
Byttneria catalpifolia is one of the most widely consumed wild species, especially those in the mountainous western part of Côte d’Ivoire. The aim of this study was to assess the nutritive potential of dried bark powder...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF DEFERIPRONE
Selective and precise RP-HPLC method was developed for estimation of Deferiprone in pure form. Acetonitrile: 0.1% formic acid (70: 30 v/v) was used as optimized mobile phase during the method development at 280 nm. Reten...
MOORVADI CHOORNA: A LITERARY REVIEW
Moorvadi choorna is herbal preparation described in the classics. It is indicated in gara visha (garopahatha pavaka) and should be given with proper anupanas like hot water, buttermilk, whey and amla rasa dravya. In pres...
VIRTUAL SCREENING FOR IDENTIFICATION OF POTENT ANTI-ASTHMATIC COMPOUNDS EXTRACTED FROM THYME PLANT:IN-SILICO APPROACH
Nowadays, asthma is a significant disease in developed and developing countries. Its chronic inflammatory lung disease. It can cause repeated symptoms such as cough, wheezing and difficulty breathing. The protein Interle...