DEVELOPMENT AND VALIDATION OF Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RIFAMPICIN AND ITS BIOENHANCER; 3’, 5-DIHYDROXYFLAVONE-7-O-Β-D-GALACTURONIDE-4’-O-Β-D-GLUCOPYRANOSIDE; IN BULK AND FORMULATION

Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2019, Vol 8, Issue 5

Abstract

The present research work demonstrates an analytical method development for simultaneous estimation of Rifampicin (RIF) and its bioenhancer; 3’,5-dihydroxyflavone-7-O-β-D-galacturonide-4’-O-β-D-glucopyranoside (CC-I) in combined dosage form using Q-absorbance ratio concept. While method development, two different wavelengths one representingIso-absorptive point (370 nm) and other representing the λmax of Rifampicin (239 nm) were used. Optimum response was obtained in solvent system that comprisesmethanol and water in ratio of 80:20 v/v.Proposed UV method was found to be linear over the concentration range of 2-20 µg/ml for Rifampicin and that of 1-24 µg/ml for CC-I.On the basis of recovery studies after standard addition, accuracy of proposed method was found to be in between 99.94 to 100.30 and 99.90 to 99.96 % for RIF and CC-I respectively. Intra-day precision of the method in terms of % relative standard deviation was found to be in between 0.21 to 1.36 and 0.21 to 1.77 for RIF and CC-I respectively. Inter-day precision range of the method for RIF and CC-I was found to be in between 0.13 to 1.94 and 0.11 to 1.58 respectively.LODand LOQ of proposed UV method were 0.043 and 0.014 µg/ml for RIF and 0.37 and 0.12 µg/ml for CC-I.Proposed UV method was robust and rugged in nature.Proposed methodwas successfully used for the estimation of RIF and CC-I contents of in-house formulation consisting of APIs and the common excipients.

Authors and Affiliations

Sachin Shivling Bhusari, Shreya Hiralal Waghmare, Pravin Shridhar Wakte

Keywords

Related Articles

FORMULATION AND EVALUATION OF CAPSULES OF SYZYGIUM CUMINI PHYTOSOMES

The objective of the study was to develop a novel Phytosome formulation of Syzygium cumini capsules by incorporation of phospholipids, for the improved permeability, solubility and better physical characteristics.The Syz...

HPTLC PROFILE OF EVEFRESH CREAM

The present study discusses about the HPTLC confirmation of the herbs used in the Siddha drug – Evefresh Cream. Method: HPTLC analysis of oil extracts of individual herbs of Evefresh Cream. Results: The study findings cl...

ANTIBACTERIALAND PHYTOCHEMICAL EVALUATIONOFLEAF EXTRACT OFMicrosorumpunctatum (l.) Copel. TOWARDS BACTERIA INVOLVED IN CUTANEOUS DISEASES

The work aimed to verifythe antibacterial and phytochemical nature of the leaves of Microsorum punctatum. The leaves of the plant wereespecially evaluated for its antibacterial capabilitywith respect to the bacteria invo...

A REVIEW ON STANDARD MILK HYGIENE PRACTICES IN INDIA WITH SPECIAL REFERENCE TO CLEAN MILK PRODUCTION

Milk is the complete food. It contains in a balanced form of all the necessary and digestible elements for building and maintaining the human and animal body. In addition, it contains immunoglobulins which protect the ne...

DRUG UTILIZATION EVALUATION AND ASSESSMENT OF ECONOMIC BURDEN OF ANTICOAGULANTS IN A TERTIARY CARE HOSPITAL IN SOUTH INDIA

Anticoagulants used for many indications, both prophylaxis and therapeutic treatment, are associated with high risk, hence drug utilization evaluation is essential. Objective: The objectives of the study were to evaluate...

Download PDF file
  • EP ID EP657825
  • DOI 10.7897/2277-4572.085152
  • Views 51
  • Downloads 0

How To Cite

Sachin Shivling Bhusari, Shreya Hiralal Waghmare, Pravin Shridhar Wakte (2019). DEVELOPMENT AND VALIDATION OF Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RIFAMPICIN AND ITS BIOENHANCER; 3’, 5-DIHYDROXYFLAVONE-7-O-Β-D-GALACTURONIDE-4’-O-Β-D-GLUCOPYRANOSIDE; IN BULK AND FORMULATION. Journal of Pharmaceutical and Scientific Innovation, 8(5), 182-188. https://europub.co.uk/articles/-A-657825