DEVELOPMENT AND VALIDATION OF Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RIFAMPICIN AND ITS BIOENHANCER; 3’, 5-DIHYDROXYFLAVONE-7-O-Β-D-GALACTURONIDE-4’-O-Β-D-GLUCOPYRANOSIDE; IN BULK AND FORMULATION
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2019, Vol 8, Issue 5
Abstract
The present research work demonstrates an analytical method development for simultaneous estimation of Rifampicin (RIF) and its bioenhancer; 3’,5-dihydroxyflavone-7-O-β-D-galacturonide-4’-O-β-D-glucopyranoside (CC-I) in combined dosage form using Q-absorbance ratio concept. While method development, two different wavelengths one representingIso-absorptive point (370 nm) and other representing the λmax of Rifampicin (239 nm) were used. Optimum response was obtained in solvent system that comprisesmethanol and water in ratio of 80:20 v/v.Proposed UV method was found to be linear over the concentration range of 2-20 µg/ml for Rifampicin and that of 1-24 µg/ml for CC-I.On the basis of recovery studies after standard addition, accuracy of proposed method was found to be in between 99.94 to 100.30 and 99.90 to 99.96 % for RIF and CC-I respectively. Intra-day precision of the method in terms of % relative standard deviation was found to be in between 0.21 to 1.36 and 0.21 to 1.77 for RIF and CC-I respectively. Inter-day precision range of the method for RIF and CC-I was found to be in between 0.13 to 1.94 and 0.11 to 1.58 respectively.LODand LOQ of proposed UV method were 0.043 and 0.014 µg/ml for RIF and 0.37 and 0.12 µg/ml for CC-I.Proposed UV method was robust and rugged in nature.Proposed methodwas successfully used for the estimation of RIF and CC-I contents of in-house formulation consisting of APIs and the common excipients.
Authors and Affiliations
Sachin Shivling Bhusari, Shreya Hiralal Waghmare, Pravin Shridhar Wakte
Keywords
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- EP ID EP657825
- DOI 10.7897/2277-4572.085152
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