DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF TELMISARTAN IN BULK AND FORMULATION USING FLUORESCENCE DETECTOR.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 2
Abstract
Asensitive, accurate and precise RP-HPLC method using Fluorescence detector has been developed and validated for the estimation of TELMISARTAN from bulk drug and Pharmaceutical Dosage form. The separation was achieved by a Prontonsil ODS analytical C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Buffer in proportion of 90:10v/v, buffer was Phosphate Buffer (pH 2.4 adjusted with Ortho Phosphoric Acid). The flow rate of mobile phase was 1.0ml/min and employing fluorescence detection with 259 nm excitation and 399 nm emission wavelengths. The retention time of TELMISARTAN was 2.6min. The calibration curve was found to be linear within the concentration range of 10ng/ml to 90ng/ml. The regression data for calibration curve shows good linear relationship with r2 = 0.9981. The method was validated in accordance with the requirements of ICH guidelines. Moreover, the proposed analytical method was applied to monitor the formulation commercially available.
Authors and Affiliations
Bhavna. A. Patel*| P.G. Department of Pharmaceutical Sciences, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India., Aanandkumari. D. Captain| A.R. and G.H. Patel College of Pharmacy, Vallabh Vidyanagar, Gujarat, India.
Keywords
Related Articles
- EP ID EP2418
- DOI -
- Views 387
- Downloads 18
HPLC Analytical Method Development and Validation Studies of Ondansetron and Rabeprazole.
Objective: Present investigation involves development and validation of chromatographic method for ondansetron and rabeprazole estimation by HPLC. Methods: The present work describes a validated reverse phase high perfor...
EFFECT OF MOMORDICA CHARANTIA L. SEEDS EXTRACT ON BIOPHYSICAL AND BIOCHEMICAL PARAMETERS OF WOUND IN EXPERIMENTALLY INDUCED DIABETES IN RATS
Momordica charantia Linn. seed extrat in the form of an ointment (20% w/w dried powder in simple ointment base) was evaluated for wound-healing potential in an excision wound model whereas the the seed extract given or...
SELF MEDICATION PATTERN AMONG MEDICAL UNDERGRADUATES IN SOUTH INDIA
Self-medication can be defined as obtaining and consuming drugs without the advice of a physician either for diagnosis, prescription or surveillance of treatment the prevalence rates are higher in the developing countrie...
GENOTOXIC IMPURITIES - AN OVERVIEW
Genotoxic impurities (GTIs) in pharmaceuticals are of increasing concerns to both pharmaceutical industries and regulatory agencies due to their carcinogenic potency for humans. Practical guidance with respect to the an...
Antimicrobial Resistance Pattern of Acinetobacter Baumannii Infection: A Comparative Study in Indoor and Outdoor Patients.
Acinetobacter baumannii is a ubiquitous pathogen that has emerged in the last few decades as a major cause of healthcare-associated infections and nosocomial outbreaks. Increasing antimicrobial resistance has been docu...