Development and Validation of RP-HPLC method for Lorazepam in Tablet Dosage Form
Journal Title: International Journal of Ayurveda and Pharmaceutical Chemistry - Year 2014, Vol 1, Issue 1
Abstract
A reversed phase-high performance liquid chromatography (RP-HPLC) method was developed for determination of Lorazepam in the tablet dosage form. The developed method was validated by measuring the parameters such as linearity range, precision, limit of detection (LOD), robustness, ruggedness, drug recovery and for the system suitability. In the HPLC method, acetonitrile: methanol (65:35 v/v) was the mobile phase, C18 column (250 mm x 4.5 mm x 5 µm), pH 4.3 and detection wavelength was 224 nm. The measured retention time of Lorazepam was found to be 4.68 minutes, which is the shortest time compared to the values reported so far. The limit of detection was 0.3µg/ml and the linearity was found to be in the range 10-100 µg/ml. The correlation coefficient value was 0.9998 and a low relative standard deviation (RSD < 1%) was obtained for linearity, precision and robustness. From the above observed parameters it can be concluded that the developed method satisfies to be a powerful tool in determination of Lorazepam drug in tablet dosage forms.
Authors and Affiliations
Dammalapati Srikantha, Rudra Raju Ramesh Raju
Keywords
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