DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ATENOLOL AND CHLORTHALIDONE FROM PHARMACEUTICAL FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 10
Abstract
A reverse phase high performance liquid chromatography (RP HPLC) method was developed and validated for the simultaneous estimation of atenolol and chlorthalidone in marketed formulation. The determination was carried out on an Xterra RP8 (150 x 4.6 mm, 5 µm) column using a mobile phase of potassium dihydrogen phosphate buffer solution: methanol (50:50v/v, pH 3.6) with flow rate 0.5ml/min (UVdetection at 240 nm). The retention time for atenolol was 3.2 min and for chlorthalidone 5.0 min. Atenolol and chlorthalidone showed a linear response in the concentration range of 50-150 µg/ml. The correlation co-efficient (' r ' value) for atenolol and chlorthalidone was 0.9996. The developed method was validated with regard to linearity, accuracy, precision, selectivity and robustness and the method was found to be precise, accurate, linear and specific. The method was validated as per ICH guidelines. The RSD for intra-day and inter-day precision were found to be less than 2 %. The percentage recoveries obtained for atenolol and chlorthalidone ranges from 100.54-103.32% and 98.03-102.77% respectively which was in good agreement with the labeled amount in the pharmaceutical formulations.
Authors and Affiliations
G. S Kumar, V. Ramya, Sumanta. Mondal, Pavan Kumar
Keywords
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