Development and Validation of RP-HPLC Method for Simultaneous Estimation of Moexipril and Hydrochlorothiazide in Bulk and Tablet Dosage Form
Journal Title: Open Access Journal of Pharmaceutical Research - Year 2017, Vol 1, Issue 7
Abstract
A simple, fast, precise reverse phase and isocratic HPLC method was developed for the separation and quantification of Moexipril (MXP) and Hydrochlorothiazide (HCT) in pharmaceutical dosage form. The quantification was carried out using Kromasil C18 (250 x 4.6 mm, 5 µm) enhanced polar selectivity column and mobile phase comprised of orthophosphoric acid buffer and acetonitrile in proportion of ratio 70:30v/v and degassed under ultra sonication. The flow rate was 1.0mL/min and the effluent was monitored at 230nm. The retention time of Moexipril and Hydrochlorothiazide were 2.4 and 3.2min respectively. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation. Linearity of Moexipril and Hydrochlorothiazide were in the range of 37.5 to 225µg/mL and 62.5 to 375µg/mL respectively. The percentage recoveries of both the drugs were 98.44% and 98.80% for Moexipril and Hydrochlorothiazide respectively from the tablet formulation. The proposed method is suitable for simultaneous determination of Moexipril and Hydrochlorothiazide in pharmaceutical dosage form.
Authors and Affiliations
Rao AL*, Prasanthi T andLatha PM
Keywords
Related Articles
- EP ID EP745882
- DOI 10.23880/oajpr-16000140
- Views 28
- Downloads 0
Review on Tropical Carriers of Antifungal Drug Therapy
Among the most prevalent conditions affecting the skin are fungus infections. OTC and oral antifungal medications are used in treatment regimens. Considering the potential negative effects of oral treatment, the topical...
Carbohydrates Test and In Vitro Inhibitory Activities of AlphaGlucosidase and Alpha-Amylase of Stem Bark Extracts of Musanga Cecropioides
Elevated postprandial hyperglycemia is one of the risk factors linked to diabetes. Decreasing postprandial hyperglycemia is possible by inhibiting certain carbohydrate hydrolyzing enzymes like α-amylase and α-glucosidase...
Exploring the Interplay of Socio-Demographic Factors, Medication Preferences, and Adherence Behaviors in Candidiasis Treatment Outcomes in Ghanaian Females
Introduction: Candidiasis, a fungal infection caused primarily by Candida species, affects millions of people worldwide, particularly women. Despite the availability of antifungal treatments, treatment failures remain a...
Assessment of Self-Medication and Disease Awareness about Migraine in Local Community of Multan, Pakistan
Background: Self-Medication and Poor disease awareness of Migraine is the furthermost public well-being concern in many countries, as well as in Pakistan. Thus considering the current epidemiological evidence about that...
Synthesis of Metal Nanoparticle Engineered Herbal Formulation for the Treatment of Skin Infections
Nanomaterials are known to be a wonder of modern medicine. A number of metal nanoparticles have been synthesized by chemical, physical and recently biological methods. Metal nanoparticles have strong inhibitory and anti...