DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE DETERMINATION OF ALVIMOPAN IN RAT PLASMA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 10
Abstract
Objective: The present investigation demonstrates a simple, sensitive and accurate high pressure liquid chromatographic (HPLC) method for the determination of alvimopan (AMP) in rat plasma.Methods: The chromatographic separation was achieved within 10 min by using acetonitrile: potassium dihydrogen phosphate buffer pH 3.0 adjusted with orthophosphoric acid (50:50) as mobile phase on Altima Grace Smart C-18 column (5μ; 250 × 4.6 mm) at a flow rate of 1.0 ml/min with injection volume 50 µl. The drug was extracted from plasma by liquid-liquid extraction using a mixture of methanol: acetonitrile (50:50) as a solvent. The retention times of drug and internal standard were found to be 5.17 and 6.74 min, respectively. This method was validated as per the United States Food and Drug Administration (US-FDA) guidelines.Results: The results of the validation parameters were found to be within the acceptance limits. The method was linear in the concentration range from 5-1000 ng/ml (r2= 0.9998), and the extraction recovery was found to be 78.71±3.86% for AMP. The lower limit of quantification was found to be 5ng/ml, and the stability of recovered samples at different conditions was found to be more than 95%.Conclusion: The developed method possess good selectivity, specificity, there was no interference found in the plasma blanks at retention times of AMP and Internal Standard (IS). We found a good correlation between the peak area and concentration of the drug under prescribed conditions. Furthermore, the method can also be used to estimate the pharmacokinetic parameters of AMP.Keywords: Alvimopan, Liquid-liquid extraction, Method development, Matrix effect, Plasma, Recovery, Stability, Validation
Authors and Affiliations
Devi Ramesh, Mohammad Habibuddin
Keywords
Related Articles
- EP ID EP566140
- DOI 10.22159/ijpps.2018v10i10.29001
- Views 85
- Downloads 0
IN VITRO ANTIBACTERIAL ACTIVITY OF PHOSPHATE ESTERS SCREENED BY BROTH DILUTION ASSAY METHOD
Objective: The present study was formulated in order to determine the novelty and the potency of the synthesized phosphate esters in terms of their antibacterial activity.Methods: Mono-6-chloro-2,4-dinitroaniline phospha...
EVALUATION OF CAFFEINE CONSUMPTION AND EFFECT DURING PREGNANCY AMONG WOMEN IN THE UAE
Objective: To investigate the relationship between caffeine consumption among pregnant women in the UAE and spontaneous abortion and stillbirth.Methods: A retrospective cross-sectional survey has been conducted on 97 pre...
PHYTOFLAVONOIDS: ANTIEPILEPTICS FOR THE FUTURE
Epilepsy has become second most common neurological disorder affecting 70 million people worldwide. Several shortcomings appeared with the use of conventional antiepileptic drugs (AEDs) like, inadequate seizure control,...
DETERMINATION OF CAPECITABINE-AN ANTICANCER DRUG IN DRIED BLOOD SPOT BY LC-ESI-MS/MS
Objective: Capecitabine (Cape), the first oral prodrug which belongs to the group of fluoro pyrimidines is the most frequently prescribed anticancer drug for the treatment of metastatic breast and colorectal cancers. The...
ANTICANCER ACTIVITIES OF THIOSEMICARBAZIDES/THIOSEMICARBAZONES: A REVIEW
There have been tremendous development in the chemotherapy of cancer and researches are still developing new and more effective drugs to combat this disease. Thiosemicarbazides and thiosemicarbazone possess a wide range...