IN VITRO ANTIBACTERIAL ACTIVITY OF PHOSPHATE ESTERS SCREENED BY BROTH DILUTION ASSAY METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 8
Abstract
Objective: The present study was formulated in order to determine the novelty and the potency of the synthesized phosphate esters in terms of their antibacterial activity.Methods: Mono-6-chloro-2,4-dinitroaniline phosphate and di-2-methyl-5-nitroaniline phosphate were screened for antibacterial activity against four pathogenic bacterial strains Staphylococcus aureus MTCC 3160, Klebsiella oxytoca ATCC 13182, Bacillus subtilis BAB 2437 and Bacillus licheniformis MS 17. Antibacterial activity was evaluated by the broth dilution assay method at different concentrations (50-2000µg/ml) of phosphate esters. Solutions of mono-and di phosphate esters were prepared in water and DMSO respectively. Growth of inoculums was noted in terms of optical density.Results: Di-2-methyl-5-nitroaniline phosphate was found more active than mono-6-chloro-2,4-dinitroaniline phosphate against selected bacterial strains. The minimum inhibitory concentration (MIC) of both phosphate esters was found in the range of 25 to 50 µg/ml. Minimum bactericidal concentration (MBC) of mono-6-chloro-2,4-dinitroaniline phosphate was found in the range of 1000 to 1500µg/ml against Staphylococcus aureus, Klebsiella oxytoca, Bacillus subtilis and Bacillus licheniformis. Di-2-methyl-5-nitroaniline phosphate showed MBC of 500 and 400 µg/ml against Staphylococcus aureus and Klebsiella oxytoca respectively, and 1000 µg/ml against Bacillus subtilis and Bacillus licheniformis. Conclusion: Both the phosphate esters have exhibited significant antibacterial activity, therefore these compounds may be a good antibacterial agent.Â
Authors and Affiliations
Mithilesh Kumari Gupta, S. K. Jadhav, S. A. Bhoite
Keywords
Related Articles
- EP ID EP578325
- DOI -
- Views 58
- Downloads 0
SYNERGISTIC EFFECT OF CURCUMA LONGA AND GLYCYRRHIZA GLABRA EXTRACTS WITH COPPER IONS ON FOOD SPOILAGE BACTERIA
Objective: To evaluate the synergistic antibacterial activity of ethanolic and aqueous extracts of Curcuma longa and Glycyrrhiza glabra in combination with copper metal ions. Methods: The phytochemical analysis of Curcum...
INTERACTION OF EBOLA VIRUS WITH INNATE AND ADAPTIVE IMMUNE SYSTEM: AN IN SILICO STUDY
Objective: Ebola virus (EBOV) is an extremely pathogenic, that can cause severe hemorrhagic fever in human with case fatality rates of up to 90% for which no vaccine or treatment is currently available. The mechanism und...
COST-EFFECTIVENESS OF BETA BLOCKERS AND THE SURVIVAL OF THE PATIENTS WITH HEART FAILURE IN THE CARDIOLOGY CENTER IN TIRANA
Objective: To evaluate the survival of the patients with heart failure in the Cardiology Center in Tirana, to evaluate the cost effectiveness of carvedilol versus metoprolol.Methods: 239 patients (pts) suffering chronic...
EFFECT OF SOLVENT EXTRACTION SYSTEM ON THE ANTIOXIDANT ACTIVITIES OF ALGAE
Objective: The present study aimed to determine the antioxidant activities of five algae including Oedogonium globossum, Pithophora oedogonia, Rhizoclonium hieroglyphicum, Scytonema ocellatum and Spirogyra communis and t...
ONLINE VISIBILITY OF PHARMACY RESEARCH IN TANZANIA: A SCIENTOMETRIC STUDY
Objective: This scientometric analysis was carried out to map the online visibility of pharmacy research at Muhimbili University of Health and Allied Sciences (MUHAS) from 1981 to 2016.Methods: Publish or Perish software...