Development and validation of RP- HPLC method for the determination of Ziprasidone in bulk and pharmaceutical dosage form

Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 1

Abstract

A simple, accurate, highly sensitive, efficient, precise and reproducible isocratic reverse phase - high performance liquid chromatographic method was developed and validated for the quantitative analysis of Ziprasidone in pharmaceutical forms. The separation was achieved on Shimadzu LC-20AT Prominence Liquid Chromatograph having a Welchrom C18 isocratic column (250 × 4.6 mm i.d., particle size 5 μm, maintained at ambient temperature) used as stationary phase. Acetonitrile: Water (50:50 v/v), adjusted to pH 3.3 with ortho phosphoric acid, and added drop wise, as mobile phase. The flow rate was set to 1.0 mL/minutes. The wavelength was determined at 251 nm using Shimadzu SPD-20A prominence UV-Vis detector. A well-defined chromatographic peak of Ziprasidone was exhibited with a retention time of 6.707 minutes and tailing factor of 1.11. Linearity was recognized for Ziprasidone in the range of 2-10 μg/mL with correlation coefficient 0.9999.Validation parameters such as specificity, linearity, precision, accuracy, and robustness, limit of detection (LOD) and limit of quantitation (LOQ) were evaluated. The projected methods were validated according to International Conference on Harmonization ICH Q2 (R1) guidelines. The developed method was successfully applied for the quantitative analysis of commercially available dosage form.

Authors and Affiliations

P. Ravisankar, K. Anusha Rani, H. Anusha, V. Himaja, S. Bhavitha, S. Gowthami

Keywords

Related Articles

A study on prescription pattern of ceftriaxone in general medicine department of a south Indian teaching hospital

A prospective observational study was conducted to assess the prescribing pattern of ceftriaxone and to determine the indication for which ceftriaxone prescribed in a general medicine department of south Indian teachin...

Comparative In- vitro evaluation of commercially available sustained release Aceclofenac tablets

Purpose: The objective of the study was to determine the difference between commercial available Aceclofenac sustained release tablets. Methods: Four commercially available tablets Hifenac SR(A), Acceclowoc SR(B), Acel...

The prescription of L-Carnitine in the treatment of Non-alcoholic fatty liver disease; A randomized and controlled clinical trial

Non-alcoholic fatty liver disease (NAFLD) consists of a range of consumption. The disease comprises clinical, Para clinical and pathological conditions from simple steatosis in non-alcoholic steatohepatitis (NASH) to f...

Phytochemical and anti diarrhoeal activity of Hippocratea africana roots

Diarrhoea is a condition that involves frequent passing of loose or watery stools. According to the Who, approximately 3.5million deaths each year are attributable to diarrhoea. Medicinal plants have been used as tradi...

The study of antihyperglycaemic activity of aqueous extract of root of Cleome chelidonii herb in rats

The work was aimed to explore the hypoglycaemic and antihyperglycaemic activity of aqueous extract of root of Cleome chelidonii herb. The extract was evaluated for its specific physical and chemical characteristics in...

Download PDF file
  • EP ID EP33365
  • DOI -
  • Views 304
  • Downloads 0

How To Cite

P. Ravisankar, K. Anusha Rani, H. Anusha, V. Himaja, S. Bhavitha, S. Gowthami (2016). Development and validation of RP- HPLC method for the determination of Ziprasidone in bulk and pharmaceutical dosage form. Indian Journal of Research in Pharmacy and Biotechnology, 4(1), -. https://europub.co.uk/articles/-A-33365