DEVELOPMENT AND VALIDATION OF PEMETREXED BY RP-HPLC METHOD IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORMS

Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 4

Abstract

A simple rapid, accurate, precise and reproducible validated reverse phase HPLC method was developed for the determination ofCeftazidimepentahydrate and Tazobactam sodium in bulk and pharmaceutical dosage forms. The quantification was carried out using Hypersil BDS C18(150 X 4.6mm, 5 µm) columnrun in isocratic way usingmobile phase comprising of phosphate buffer pH 3.0, acetonitrile, and tetrahydrofuran in the ratio of 60:30:10 with a detection wavelength of 205nm and injection volume of 20µL, with a flow rate of 1.0ml/min. The retention timesof the drugs were found to be 3.490min and 2.353min. The linearity ranges of the proposed method lies between 60-140mcg/ml and 7.5-17.5mcg/ml for Ceftazidimepentahydrate and Tazobactam sodium with correlation coefficient of r2=0.999 for both. The assay of the proposed method was found to be 99.38% and 99.26%. The recovery studies were also carried out and mean % Recovery was and found to be 99.91% and 100.84%. The % RSD from reproducibility was found to be <2%.The proposed method was statistically evaluated and can be applied for routine quality control analysis of Ceftazidimepentahydrate and Tazobactam sodium in bulk and in Pharmaceutical dosage form.

Authors and Affiliations

Suresh Kumar Agrawal1, Devendra Singh Rathore

Keywords

Related Articles

Formulation and evaluation of diclofenac sodium S.R. tablets using Linum usitatissimum seed mucilage Matrixing

The main accusative of the present work was to develop sustained release matrix tablets of Diclofenac sodium for maintaining therapeutic blood or tissue levels of the drug for extended period of time with minimized loc...

Analytical method development and validation for the simultaneous estimation of Levamisole and Mebendazole in bulk & tablet formulation by RP-HPLC method

A fast, robust and accurate RP-HPLC method was developed and validated for simultaneous determination of Levamisole and Mebendazole in tablets. The mobile phase was mixture of aqueous phosphate buffer with pH 5.2: Meth...

Anticonvulsant activity of alcoholic extract of fruits of Capsicum annuum

The anticonvulsant activity of ethanolic extract of fruits of Capsicum annuum was evaluated in albino mice. The ethanolic extract of fruits of Capsicum annuum (50mg/kg), was studied for their anticonvulsant effect on m...

Efficacy and tolerability of corticosteroids in pediatric cancer chemotherapy patients

Efficacy and tolerability in the treatment of pediatric cancer was studied in the present investigation. Prednisone, Hydrocortisone, Dexamethasone, Prednisolone and Methyl prednisolone were used in the treatment. The p...

Evaluation of the hepatoprotective activity of Rhus mysorensis in albino rats

The whole plant of Rhus mysorensis of Anacardiaceae family is traditionally used in the treatment of diabetes. The aim of the present study was to evaluate the ethanolic extract of the whole plant of Rhus mysorensis fo...

Download PDF file
  • EP ID EP33095
  • DOI -
  • Views 428
  • Downloads 0

How To Cite

Suresh Kumar Agrawal1, Devendra Singh Rathore (2013). DEVELOPMENT AND VALIDATION OF PEMETREXED BY RP-HPLC METHOD IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORMS. Indian Journal of Research in Pharmacy and Biotechnology, 1(4), -. https://europub.co.uk/articles/-A-33095