Development and validation of new RP-HPLC method for simultaneous estimation of drug Thiocolchicoside and Etodolac in tablet dosage form
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 4
Abstract
A simple, economic, accurate and precise reverse phase high performance liquid chromatography method for analysis of Thiocolchicoside and Etodolac was developed and validated according to ICH guidelines. The quantification of the drug was carried out using PDA (photodiode array) detector. Column in isocratic mode, with mobile phase Methanol:Buffer in ratio of 60:40 was used. The flow rate was 1.0 ml/min and effluent was monitored at 254nm.the retention times were 2.869 and 3.942 min for Thiocolchicoside and Etodolac respectively. The injection volume was 20µl.as per ICH guidelines the method was validated and the method was found to be linear in the range of 20-80µg/ml for Thiocolchicoside and Etodolac. Percent recovery studies of Thiocolchicoside and Etodolac 99.62%and99.74%. The limit of detection and quantification was found to be 0.25&0.77µg/ml Thiocolchicoside 0.34&1.05µg/ml for Etodolac. The values of precession and robustness lie within the acceptance limit. Thus the proposed method can be successfully applied for simultaneous determination of Ceftriaxone and Vancomycin in routine analysis work.
Authors and Affiliations
Sampasavu Syamala
Keywords
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