Direct shoot organogenesis from leaf explants of Aquilaria malaccensis Lam
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2015, Vol 3, Issue 2
Abstract
The shoot regeneration in Aquilaria malaccensis was mainly been achieved from shoot tips or nodal explants. In this paper, the direct shoot organogenesis from leaf explants of agar tree has been presented. The juvenile leaf explants of agar tree were inoculated on MS medium supplemented with varying levels of BAP (0-2mg/l) + NAA (0.05-0.4 mg/l), many bud like protuberances appeared at the leaf tips. The period required for bud induction was 38 days. The highest induction percentage (53.33%) was recorded in the media supplemented with 2mg/l BAP+ 0.1mg/l NAA as compared to other treatments. The high cytokinin low auxin ratio positively influenced direct organogenesis in leaf explants of this tree species. The root induction percentage was recorded highest in half strength MS (½MS) medium supplemented with 1mg/l NAA as compared to other concentrations and combinations of hormones. Roots were not formed in hormone free MS medium. This is the first report on direct regeneration of the whole plant of A. malaccensis from leaves.
Authors and Affiliations
MoitreyeeSaikia and KarunaShrivastava
Keywords
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