Terminalia bellerica leaf extracts induce apoptosis in Hep G2 cells and regulates cell cycle progression by inducing G2/M cell cycle arrest
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2014, Vol 2, Issue 1
Abstract
The present study implicit the observation that leaf extracts of Terminalia bellerica have promising anticancer ingredients with IC50 of 5.65µg/ml. Phytochemical studies have revealed that Terminalia bellerica contains a variety of bioactive ingredients with reported therapeutic activities. Methanolic Terminalia Leaf Extracts (MTLE) demonstrated a dose dependent growth inhibitory activity with a certain degree of selectivity against cell line Hep G2 cell line. MTLE supplementation down regulated expression of Bcl-2 a potent suppressor of apoptosis and induced expression of pro apoptotic proteins Bax, caspase- 9 and caspase-3 in a dose dependent manner. We also demonstrated that the pro apoptotic effect of MTLE was due to down regulation of the Akt/mTOR signaling pathway. Flow cytometry analysis showed an increase in the percentage of G2/M arrest phase in Hep G2 cell line compared to control cell line. In summary our findings demonstrate that MTLE treatment of the Hep G2 cells resulted in significant (a) cell growth inhibition, (b) G2/M-phase cell cycle arrest, and (c) apoptosis in a dosedependent fashion by triggering caspase-9 and caspase-3 activation. Overall, these findings suggest that leaves of Terminalia can provide a source of potential therapeutic compounds for the treatment of cancer.
Authors and Affiliations
Dinesh M. G, Rajasekaran Subbarayan, Suneel Rallapalli Chandrasekaram Kansrajh, R. Kalaivani
Keywords
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