Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Aceclofenac and Thiocolchicoside in Tablet Dosage Form
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2014, Vol 3, Issue 1
Abstract
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Aceclofenac and Thiocolchicoside in tablet dosage form. Chromatographic analysis was performed on a Symmetry Nucleosil C8 (150X4.6 mm,5µm) column ambient temperature with a mixture of mixed phosphate buffer and Acetonitrile in the ratio 40:60(buffer preparation: Prepare 0.01M Disodium hydrogen orthophospate as mobile phase, at a flow rate of 0.80 mL min-1. UV detection was performed at 261 nm. The method was validated for accuracy, precision, specificity, linearity and sensitivity. The retention times of Aceclofenac and Thiocolchicoside were 2.167 and 4.866 min, respectively. The Limit of detection was 0.33 and 3.9 µg mL-1 and the quantification limit was 1.002 µg mL-1 and 11.9 µg mL-1 for Aceclofenac and Thiocolchicoside respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.94% to 99.81%.
Authors and Affiliations
S Venkata Subba Reddy
Keywords
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