DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF LEVOFLOXACIN HEMIHYDRATES AND CEFIXIME TRIHYDRATE IN MARKETED FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
A simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method has been developed for the simultaneous determination of Levofloxacin hemihydrates and Cefixime trihydrate from tablet dosage forms using C18 column (¬Grace Smart (250mm x 4.6mm, 5µm). The sample was analyzed using 0.08M Potassium dihydrogen phosphate: Acetonitrile in the ratio of 60:40 (%v/v) pH adjusted to 6.5 with triethylamine, as a mobile phase at a flow rate of 1.0ml/min and detection at 254nm. The retention time for Cefixime trihydrate and Levofloxacin hemihydrates was found to be 2.19min and 3.60min respectively. The method can be used for estimation of combination of these drugs in tablets. The method was validated as per ICH guidelines. The linearity of developed method was achieved in the range of 20-120μg/mL (r2=0.9995) for Cefixime trihydrate and 20-120μg/mL (r2=0.9995) for Levofloxacin hemihydrates and assay of tablets were between 98.0-102.0%. Due to these attributes, the proposed method could be used for routine quality control analysis of these drugs in combined dosage forms.
Authors and Affiliations
Vipul Dhandhukiya , Priya Tiwari , Vijay Godavariya
Keywords
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- EP ID EP98661
- DOI 10.7897/2230-8407.04618
- Views 116
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