Development and validation of simple and rapid LC-MS/MS method for ezetimibe in human plasma and its application to bioequivalence study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 17, Issue 2
Abstract
A sensitive and specific liquid chromatography combined with electrospray ionization (ESI) tandem mass spectrometry (LC-MS/MS) method, operating in the negativeionization mode, for quantifying of ezetimibe in human plasma using ezetimibe-d4 as internal standard (IS) was developed and validated. The analyte and IS were extracted by simple one step liquid to liquid extraction (LLE). The chromatographic separation was performed on a Gemini C18 column (50 X 2.0 mm, 5 um) under isocratic conditions using a mixture of acetonitrile/0.1% formic acid (70:30, v/v) as mobile phase at a flow rate of 0.20 ml/min. Quantitation was performed using multiple reaction monitoring (MRM) mode to study parent → product ion transitions of m/z 408.0→270.8 for ezetimibe and m/z412.1→270.8 for IS, respectively. Linearity in plasma was obtained over the concentration range 0.1 ~ 20 ng/ml, with a coefficient of determination (r2 ) of 0.9999. The method was has been successfully applied for routine assay to support pharmacokinetic study of ezetimibe in human plasma after an oral administration of 10 mg ezetimibe.
Authors and Affiliations
Yong Sun Park, Dong Pil Kimb, Suk Hong Kang, Ki Tak Kimb ,, Yong Hwan Kim, Jong Hoon Kim, Ye Na Kim, Jin Young Kima, Soo Ji Sim, Bo Ra Jin, Heon Soo Leea
Keywords
Related Articles
- EP ID EP390311
- DOI 10.9790/3008-1204070108
- Views 73
- Downloads 0
A Study of Indications, Type and Complications of Tracheostomy in a Teritiary Referral Hospital.
AIMS 1.To identify the common indications for tracheostomy. 2. To know the type of tracheostomy required 3. To study the complications in tracheostomy MATERIALS AND METHODS: This study was conducted in the Government ENT...
Patch graft following perforation in Terrien’s Marginal degeneration
PURPOSE: To report a case of patch graft performed for corneal perforation in a patient with Terrien’s marginal degeneration. METHOD: A young woman presented with sudden painless loss of vision following a trivial trauma...
Materials Used for Maxillofacial Prosthesis: A Review
Congenital and acquired defects of the face create an unfortunate condition for the affected individual. To lead a comfortable life patient requires facial rehabilitation. To perform rehabilitation of such patients, reas...
Antimicrobial Activity of MTA Fillapex, Real Seal SE, Acroseal and Zinc oxide Eugenol Sealers against Enterococcus Faecalis and Candida Albicans
Aim:The aim of the present study was to evaluate the antimicrobial activity of different root canal sealers (MTA Fillapex, Real Seal SE, Acroseal and Zinc Oxide Eugenol) against Enterococcus fecalis and Candida albicans....
Malarial Perspective of the Kalita Community of Dhemaji and Dibrugarh District, Assam
Background: Malaria is one of the major public health problems in the Northeast India. Ignorance, low awareness and not prioritizing health are some of the issues in early identification and prompt treatment of diseases....