DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF DIAZEPAM AND PROPRANOLOL HYDROCHLORIDE IN COMBINED DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 8
Abstract
The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of diazepam and propranolol hydrochloride in combined tablet dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra was obtained in 0.05M methanolic sulphuric acid and the determinations were made at 248 nm (ZCP of propranolol hydrochloride) for diazepam and 242 nm (ZCP of diazepam) for propranolol hydrochloride. The linearity was obtained in the concentration range of 2.5-30 μg/ml for both diazepam and propranolol hydrochloride. The mean recovery was 99.77 ± 1.39 and 100.6 ± 1.18 % for diazepam and propranolol hydrochloride, respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of diazepam and propranolol hydrochloride in pharmaceutical tablet dosage form. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Satish Patel , Paresh Patel , Natavarlal Patel
Keywords
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