DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF GLICLAZIDE AND SITAGLIPTIN PHOSPHATE MONOHYDRATE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: The present study describes a simple, accurate, precise, specific and economical UV spectrophotometric method for simultaneous estimation of Gliclazide (GLZ) and Sitagliptin phosphate monohydrate (SPM) in bulk and pharmaceutical dosage form. Methods: The method was validated in terms of linearity, sensitivity, accuracy, precision, limit of detection and limit of quantification. The solvent used was methanol and the absorption maxima for Gliclazide (GLZ) and Sitagliptin phosphate monohydrate (SPM) were found to be 226 nm and 267 nm respectively. Results: The percentage recovery of Gliclazide (GLZ) and Sitagliptin phosphate monohydrate (SPM) were 99.8% and 99.64% respectively. The linear response was observed in the range of 7-27 µg/ml and 20-100 µg/ml with a correlation coefficient (r2) of 0.996 and 0.998 for Gliclazide (GLZ) and Sitagliptin phosphate monohydrate (SPM) respectively. Conclusion: The proposed method was successfully applied for the quantitative detection of Gliclazide (GLZ) and Sitagliptin phosphate monohydrate (SPM) in Pharmaceutical dosage form.Â
Authors and Affiliations
C. Rubina Reichal, M. Gopal Rao
Keywords
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