DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF OXYCLOZANIDE AND LEVAMISOLE HCl
Journal Title: World Journal of Pharmaceutical and Medical Research - Year 2017, Vol 3, Issue 4
Abstract
A simple, rapid validated stability indicating HPTLC method for simultaneous estimation of Oxyclozanide and Levamisole HCl was successfully developed. This method is based on HPTLC separation followed by UV detection at 220 nm. The separation was carried out on Merck TLC aluminium sheets precoated with silica gel 60F254 using Ethyl acetate: Ammonia: TEA: Methanol (6:0.5:0.2:0.5 v/v) as a mobile phase. Oxyclozanide and Levamisole HCl gave well defined and sharp peak at Rf 0.40 ± 0.02 and 0.80 ± 0.02 at 220 nm. Calibration curve was linear in range 100-500ng/band for Oxyclozanide and 200-1000ng/band for Levamisole HCl. Stress degradation study includes acid and base hydrolysis, oxidation, and thermal and photolytic stress conditions. This method can be applied to determination of stability of Oxyclozanide and Levamisole HCl. The suitability of this HPTLC method for quantitative determination of Oxyclozanide and Levamisole HCl was proved by validation in accordance with requirements of ICH guidelines Q2A (R1).
Authors and Affiliations
Mrinalini C. Damle
Keywords
Related Articles
- EP ID EP664653
- DOI -
- Views 155
- Downloads 0
MALE-INFERTILITY: IS ANTIHISTAMINIC ONE OF THE CAUSE?
We all know that allergy is common in developed countries and antihistaminic are common drugs used for symptomatic relief; the drug which might have impact on male fertility. So, A study is needed to be conducted on huma...
ROLE OF ULTRASOUND IN IMPROVING THE MICROBIOLOGICAL QUALITY OF DRINKING WATER
Water is the most essential element in this world to sustain life, and an adequate, safe and accessible water supply must be made available to all. Improving access to safe drinking-water can result in tangible benefits...
FREQUENCY OF HYPOCALCAEMIA AMONG FULL TERM NEONATES WITH SEVERE BIRTH ASPHYXIA
Although neonates with severe birth asphyxia are known to be at increased risk of early onset hypocalcaemia, the magnitude of problem is not well documented. The objective of this study was to determine the prevalence of...
RASA DRAVYA AND THEIR USE IN THE MANAGEMENT OF LIFE STYLE DISEASES: AN AYURVEDA PERSPECTIVE
Rasa Shastra, an integral part of Ayurveda deals with Rasa (mercury) and other minerals, metals, herbal poisonous plants and aquatic origin substances. The Rasa aushadhi so prepared offers potent immunization, has rejuve...
ROLE OF IDEAL NIDRA IN MAINTAINING GOOD HEALTH: AN AYURVEDIC REVIEW
Millions of people are yearly dying due to unhealthy conditions. Even with the advent of excellent techniques and astonishing advancement in medical science and technology, the humanity is left with innumerable health pr...