DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BECLOMETHASONE DIPROPIONATE AND SALBUTAMOL SULPHATE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: To develop a validated stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method for simultaneous estimation of Beclomethasone dipropionate (BEC) and Salbutamol sulphate (SAL) in bulk and combined dosage form.Methods: An isocratic, RP-HPLC method was developed using Hi Q Sil C18 (250 x 4.6 mm, 5 µm) column and 1 mM ammonium acetate buffer and methanol (15:85 v/v) as mobile phase at flow rate of 1 ml/min and detection wavelength of 230 nm.Results: The chromatographic conditions yield good separation between drugs with retention time (RT) of 7.75±0.03 min and 2.36±0.09 min, for BEC and SAL, respectively. The method was validated with respect to linearity, precision, accuracy and robustness. The data of linear regression analysis indicated a good linear relationship over the range of 5–30 µg/ml concentrations with a correlation coefficient (r2) of 0.997 for both the drugs.Conclusion: The developed method was found to be simple, sensitive, selective, accurate, and repeatable for simultaneous analysis of BEC and SAL and can be adopted for routine analysis of these drugs in bulk and pharmaceutical dosage form.Â
Authors and Affiliations
Santosh V. Gandhi, Prashant S. Mittal, Ashwini M. Gaikwad
Keywords
Related Articles
- EP ID EP578697
- DOI -
- Views 74
- Downloads 0
SYNTHESIS OF SOME NOVEL 3,5,6-TRISUBSTITUTED-[1,2,4]TRIAZOLO[3,4-c][1,2,4] TRIAZINESAS DNA PHOTOCLEAVING AGENTS
Objective: To develop an easy and non toxic method for synthesis of some novel triazolotriazines via oxidative transformation of triazinylhydrazones using iodobenzene diacetate and to evaluate their DNA photocleavage pot...
MEDICINES WASTE POLICIES AND THE POPULATION KNOWLEDGE IN BRAZIL
Objective: To investigate the degree of knowledge of the patients enrolled in the Unified Health System of the Medicine School of ABC (Faculdade de Medicina do ABC) regarding the proper use and disposal of medicaments....
THE ROLE OF HONEY WITH ROYAL JELLY IN PROTECTING THE GRAAFIAN FOLLICLES FROM THE TOXICITY OF THE ADRIAMYCIN DRUG
Objective: This study aims to investigate the ability of certain mixture of honey with royal jelly (H+R) to prevent the toxic side effects, caused by the Adriamycin drug. One of the most important side effects of this dr...
RETROSPECTIVE STUDY ON THERAPEUTIC DRUG MONITORING OF LAMOTRIGINE IN INDIAN EPILEPTIC PATIENTS
Objective: Antiepileptic drugs (AED) are administered either singly or in combination with other drugs. Their pharmacokinetics is influenced by drug-drug interaction & inter-individual variations. Lamotrigine (LTG) i...
IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT
Objective: The present study aims to investigate in vitro anthelmintic and anti-amylase properties of the ethanolic fruit extract of Garcinia pedunculata.Methods: For the study, mature fruits of G. pedunculata were colle...