IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 1
Abstract
Objective: The present study aims to investigate in vitro anthelmintic and anti-amylase properties of the ethanolic fruit extract of Garcinia pedunculata.Methods: For the study, mature fruits of G. pedunculata were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of G. pedunculata was used for evaluation of in vitro anthelmintic and anti-amylase activities. In vitro anthelmintic activity was evaluated in animal models, Pheretima posthuma, an earthworm species. In vitro anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].Results: Ethanolic extract of G. pedunculata showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of G. pedunculata showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.Conclusion: It could be concluded that ethanolic extract of G. pedunculatahas biological properties which could be utilised in medicine by characterising its bioactive components.Â
Authors and Affiliations
Pinky Sarmah, Nako Kobing, Jyotchna Gogoi, Ananta Madhab Dutta
Keywords
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- EP ID EP575792
- DOI 10.22159/ijpps.2017v9i1.15395
- Views 57
- Downloads 0
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