Development and Validation of UV Spectroscopic and HPTLC Methods for the Determination of Bosentan from Tablet Dosage Form
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 2
Abstract
Two simple, sensitive and precise UV spectroscopic and HPTLC methods were developed and validated for the determination of bosentan in tablet dosage form. UV spectroscopic method: For the determination of bosentan, the stock solution of 100 μg/ml of drug was prepared in dichloromethane. The measurement of absorbance was noted at 274 nm. The calibration curve was obtained in the range 10-40 μg/mL. The slope, intercept and correlation coefficient values were found to be 0.02975, -0.0063 and 0.9992, respectively. HPTLC method: For the determination of bosentan by HPTLC method, a precoated silicagel 60 F254 on aluminium sheets and a mobile phase system comprising of methanol: ammonia (10: 1 drop, v/v) were selected. After development the plate was scanned and quantified at 288 nm. Linearity was found in the concentration range of 10 to 80 ng/spot (r = 0.9991). Limit of detection was found to be 1 ng/spot and limit of quantification was found to be 4 ng/spot. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise, accurate and free from interference present in formulation. Keywords: Bosentan, HPTLC, UV spectroscopy, Method Validation, ICH guidelines
Authors and Affiliations
A. Suganthi ,, Sai Lakshmi. C. H, Sruth i Vinod, T. K. Ravi ,
Keywords
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