Development and Validation of UV Spectroscopic and HPTLC Methods for the Determination of Bosentan from Tablet Dosage Form
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 2
Abstract
Two simple, sensitive and precise UV spectroscopic and HPTLC methods were developed and validated for the determination of bosentan in tablet dosage form. UV spectroscopic method: For the determination of bosentan, the stock solution of 100 μg/ml of drug was prepared in dichloromethane. The measurement of absorbance was noted at 274 nm. The calibration curve was obtained in the range 10-40 μg/mL. The slope, intercept and correlation coefficient values were found to be 0.02975, -0.0063 and 0.9992, respectively. HPTLC method: For the determination of bosentan by HPTLC method, a precoated silicagel 60 F254 on aluminium sheets and a mobile phase system comprising of methanol: ammonia (10: 1 drop, v/v) were selected. After development the plate was scanned and quantified at 288 nm. Linearity was found in the concentration range of 10 to 80 ng/spot (r = 0.9991). Limit of detection was found to be 1 ng/spot and limit of quantification was found to be 4 ng/spot. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise, accurate and free from interference present in formulation. Keywords: Bosentan, HPTLC, UV spectroscopy, Method Validation, ICH guidelines
Authors and Affiliations
A. Suganthi ,, Sai Lakshmi. C. H, Sruth i Vinod, T. K. Ravi ,
Keywords
Related Articles
- EP ID EP377448
- DOI -
- Views 107
- Downloads 0
In vitro Cytotoxic Activity of Luffa acutangula on Human Neuronal Glioblastoma and Human Lung Adenocarcinoma Cell Lines
In vitro studies were carried out to evaluate the cytotoxic potential of the ethanolic and aqueous extracts of Luffa acutangula against human neuronal glioblastoma cells (U343) and human lung cancer cells (A549). Screeni...
Pharmaceutical Marketing Communication Strategies and Tools; Analysis of influence over physician’s prescribing preferences
Pharmaceutical companies adopt different marketing communication strategies and tools in order to influence physician’s prescriptions in their favor. These communication strategies and tools play a very important role in...
Phytopharmacological Review of Plumeria species
The various species of Plumeria are known to have medicinal properties and have a long history of use by indigenous and tribal people in India. The medicinal value of this Plumeria species in the treatment of a large nu...
Biological and Chemical Evaluation of Leaf Extracts of Dalbergia saxatilis Hook F. (Fabaceae)
The air-dried powdered leaf of Dalbergia saxatil is was extracted with 95% ethanol to obtain the crude extract which was fractionated into acidic, basic, non-polar and polar neutral fractions. The crude ethanol extract e...
Evaluation of Hypoglycemic Effect of Crude Extracts and Different Fractions of Stem Bark of Acacia nilotica
Diabetes mellitus is a multifactorial disease which is characterized by hyperglycemia, lipoprotein abnormalities, raised basal metabolic rate, defect in reactive oxygen species scavenging enzymes and altered intermediary...