DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF LAMIVUDINE AND ZIDOVUDINE IN BULK AND TABLET DOSAGE FORM
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2017, Vol 7, Issue
Abstract
Abstract Simple, rapid, sensitive, precise and specific UV spectrophotometric method for the determination of Lamivudine (LAMI) and Zidovudine (ZIDO) in bulk drug and tablet dosage form were developed and validated. A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. UV-visible spectrophotometric method, measurement of absorption at maximum wavelength in 10 ml acetonitrile and volume make with water solvent system as reference LAMI and ZIDO were found to be at 271 nm and 262 nm respectively. The drug obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 2-10 μg/ml for LAMI and 1- 5μg/ml for LAMI respectively with correlation coefficient was 0.999. The LOD and LOQ of LAMI were found to be 0.3831(μg/mL) and 1.1609 (μg/ml), ZIDO were found to be 0.6276μg/ml) and 1.9018 (μg/ml), respectively. The proposed method is precise, accurate and reproducible and can be used for routine analysis of LAMI and ZIDO in bulk and tablet dosage form.
Authors and Affiliations
Lokendra Bhamare*, Sufiyan Ahmad, V. M. Shastry
Keywords
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