DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF KETOPROFEN AND THIOCOLCHICOSIDE IN SOLID ORAL DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 5
Abstract
A simple, accurate, precise, economical and cost-effective UV Spectrophotometric method has been developed for simultaneous estimation of Ketoprofen and Thiocolchicoside in solid oral dosage form and validated as per International Conference of Harmonisation guidelines. Estimation of Ketoprofen and Thiocolchicoside was conducted by using simultaneous equation method based on measurement of absorbance at two wavelengths. The solvent used was methanol and the λmax of Ketoprofen and Thiocolchicoside were found to be 251.5 nm and 372 nm respectively. At 251.5 nm both the drugs have considerable absorbance. The linearity was found in the range of 5-25 μg/ml and 4-20 μg/ml for Ketoprofen and Thiocolchicoside respectively. The linearity coefficient was found to be 0.995 at 251.5 nm for Ketoprofen and 0.986 and 0.996 at 251.5 nm and 372 nm for Thiocolchicoside respectively. Recovery study values of Ketoprofen and Thiocolchicoside was found to be 99.84% and 100.57% respectively. % RSD for intra-day and inter-day precision studies was found to be less than ± 2. The Limit of Detection and Limit of Quantitation were found to be 16.58 μg/ml and 50.25 μg/ml for Ketoprofen and 13.25 μg/ml and 40.16 μg/ml for Thiocolchicoside respectively. The proposed method was satisfactorily validated as per the ICH guidelines and can be successfully employed for routine analysis of Ketoprofen and Thiocolchicoside in solid oral dosage form.
Authors and Affiliations
Ankita Bhavsar , Toral Joshi , Kartik Vikani , Arvind Senta
Keywords
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- EP ID EP154172
- DOI 10.7897/2230-8407.07552
- Views 103
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