Abstract

Abstract The current research aimed to develop and validate a simple, accurate and sensitive analytical method using spectrophotometer device in assaying raw material of atenolol and determination the content of atenolol in pharmaceutical tablets. Firstly, a solution of atenolol in hydrochloric acid (0.1N) was prepared and its absorption spectrum was examined in UV range against blank solution containing only hydrochloric acid (0.1N). The method was validated for linearity through preparation a series of certain concentration and measure their absorptions and correlation coefficient ,molar absorptivity, and Sandell's sensitivity were calculated. Method was detected for accuracy by performing recovery studies. The precision of the method was expressed as RSD of series of measurement by replicate estimation of drug. The proposed method was both evaluated for specificity by noticing any effects of common excipients that may add to atenolol pharmaceutical formulation and applied to commercial tablets The results indicated that the solution of atenolol in hydrochloric acid (0.1N) exhibiting λ max at 225 nm. The relationship between the concentrations and the absorbance was linearity with good correlation coefficient (0.9979) The molar absorptivity is 7.163×103Lmol-1cm-1and Sandell's sensitivity is 0.034μg/cm2. The method is appropriate over concentration range of 5 – 40μg/ml. The results of studying the validation of this method showed that the method have been validated statistically. The proposed method had its accuracy, precision, specificity, when applied in measurement atenolol in its pharmaceutical dosage forms. As a result we can use the proposed analytical method in determination atenolol in raw material and its commercial tablets.

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