Development of a GC-MS Assay for the Determination of Fentanyl Pharmacokinetics in Rabbit Plasma after Sublingual Spray Delivery
Journal Title: The AAPS Journal - Year 2008, Vol 10, Issue 2
Abstract
Fentanyl free base was kindly supplied by Insys Therapeutics, Inc (Mundelein, IL), fentanyl citrate and sufentanil free base were obtained from Mallinckrodt Chemicals (St. Louis, MO). 1-Chlorobutane was purchased from Acros Organics (New Jersey), methanol and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ). SurfaSil™ siliconizing fluid was obtained from Pierce (Rockford, IL), hydrogen peroxide 3% USP solution from Hydrox Labs (Elgin, IL), bacteriostatic sodium chloride injection USP 0.9% from APP (Schaumburg, IL), and AErrane™ isoflurane gas was provided by VMC Anesthesia Ohmeda (Waukesha, WI). Heparinized caraway capillary tubes were purchased from Baxter Healthcare Corporation (McGraw Park, IL). Tuberculin slip tip sterile catheters were purchased from J&J Medical (New Brunswick, NJ). All vials, crimp caps with PTEF/silicone septa, and siliconized micro centrifuge tubes, were purchased from Fisher Scientific (Fair Lawn, NJ). Water for HPLC use was passed through a reverse osmosis system (Milli-Q® Reagent Water System) before use. New Zealand rabbits were purchased from Myrtle’s Rabbitry, Inc. (Thompson Station, TN).
Authors and Affiliations
Ahmad H. Malkawi, Abeer M. Al-Ghananeem, Peter A. Crooks
Keywords
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- EP ID EP681554
- DOI 10.1208/s12248-008-9031-x
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Holford NHG and Sheiner LB “Understanding the Dose-Effect Relationship-Clinical Application of Pharmacokinetic-Pharmacodynamic Models”, Clin Pharmacokin 6:429–453 (1981)—The Backstory