Development of a GC-MS Assay for the Determination of Fentanyl Pharmacokinetics in Rabbit Plasma after Sublingual Spray Delivery
Journal Title: The AAPS Journal - Year 2008, Vol 10, Issue 2
Abstract
Fentanyl free base was kindly supplied by Insys Therapeutics, Inc (Mundelein, IL), fentanyl citrate and sufentanil free base were obtained from Mallinckrodt Chemicals (St. Louis, MO). 1-Chlorobutane was purchased from Acros Organics (New Jersey), methanol and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ). SurfaSil™ siliconizing fluid was obtained from Pierce (Rockford, IL), hydrogen peroxide 3% USP solution from Hydrox Labs (Elgin, IL), bacteriostatic sodium chloride injection USP 0.9% from APP (Schaumburg, IL), and AErrane™ isoflurane gas was provided by VMC Anesthesia Ohmeda (Waukesha, WI). Heparinized caraway capillary tubes were purchased from Baxter Healthcare Corporation (McGraw Park, IL). Tuberculin slip tip sterile catheters were purchased from J&J Medical (New Brunswick, NJ). All vials, crimp caps with PTEF/silicone septa, and siliconized micro centrifuge tubes, were purchased from Fisher Scientific (Fair Lawn, NJ). Water for HPLC use was passed through a reverse osmosis system (Milli-Q® Reagent Water System) before use. New Zealand rabbits were purchased from Myrtle’s Rabbitry, Inc. (Thompson Station, TN).
Authors and Affiliations
Ahmad H. Malkawi, Abeer M. Al-Ghananeem, Peter A. Crooks
Keywords
Related Articles
- EP ID EP681554
- DOI 10.1208/s12248-008-9031-x
- Views 55
- Downloads 0
DARPP-32 mediates the actions of multiple drugs of abuse
Drugs of abuse share the ability to enhance dopaminergic neurotransmission in the dorsal and ventral striatum. The action of dopamine is modulated by additional neurotransmitters, including glutamate, serotonin and adeno...
Issues related to targeted delivery of proteins and peptides
While modern genomic and proteomic technology enables rapid screening of novel proteins and peptides as potential drug candidates, design of delivery systems for these biologics remains challenging especially to achieve...
Bladder Tissue Pharmacokinetics of Intravesical Mitomycin C and Suramin in Dogs
Suramin, at non-cytotoxic doses, reverses chemoresistance and enhances the activity of mitomycin C (MMC) in mice bearing human bladder xenograft tumors. The present study evaluated the pharmacokinetics of the intravesica...
Enhancement of MHC-I Antigen Presentation via Architectural Control of pH-Responsive, Endosomolytic Polymer Nanoparticles
The online version of this article (doi:10.1208/s12248-014-9697-1) contains supplementary material, which is available to authorized users.
Reproductible production of a PEGylated dual-acting peptide for diabetes
A pEGylated glucagon-like peptide-1 (GLP-1) agonist and glucagon antagonist hybrid peptide was engineered as a potential treatment for type 2 diabetes. To support preclinical development of this PEGylated dual-acting pep...