Development of a New Approach for Standardization of the Herb Centaurium erythraea Rafn. by High Performance Liquid Chromatography
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2020, Vol 17, Issue 6
Abstract
Objectives: The aim of this study was the development a new, fully validated high performance liquid chromatography (HPLC) method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the herb Centaurium erythraea Rafn. The article describes a new approach to the standardization of C. erythraea and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by swertiamarin. Materials and Methods: The quantitative determination of swertiamarin was performed in isocratic mode on a Symmetry C18 column using water and acetonitrile as solvents for the mobile phase. Results: Validation characteristics of the developed method showed that it was linear in the whole range of concentrations from 0.01 mg/mL to 0.05 mg/mL swertiamarin. All validation characteristics met the established acceptance criteria. Conclusion: This method can be used in the standardization of raw materials, as well as in the analysis of medicinal products and dietary supplements that include C. erythraea. The established chromatographic method was successfully applied for the analysis of raw materials of C. erythraea with the quantitative content determination of swertiamarin in the analyzed samples.
Authors and Affiliations
Svitlana M. GUBAR, Anna S. MATERIIENKO, Nataliia M. SMIELOVA, Liana G. BUDANOVA, Victoriya A. GEORGIYANTS
Keywords
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