Effect of Probucol on Proliferation of Leukemia, Multiple Myeloma, Lymphoma, and Fibroblast Cells
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2021, Vol 18, Issue 1
Abstract
Objectives: Probucol is a bisphenol antioxidant with antiinflammatory, antilipidemic and antidiabetic effect. Development and progression of cancer is closely related to chronic inflammation and oxidative stress. Agents that target these processes have been shown to modulate cancer cell proliferation. In this regard, the effect of probucol on proliferation of different cancer cell lines was investigated. Materials and Methods: Different concentrations of probucol solutions were prepared and applied to the following cancer cell lines: K562S (imatinib sensitive) and K562R (imatinib resistant) chronic myeloid leukemia (CML) cells; U937 histiocytic lymphoma cells; HL60 acute myeloid leukemia cells; U266, H929, and RPMI8226 multiple myeloma cells; and L929 fibroblast cells. Cell viability was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: Significant toxicity was not exhibited due to probucol treatment (0.1-10 µM) in K562S and K562R CML cells, U937 histiocytic lymphoma cells, HL60 acute myeloid leukemia cells, U266 multiple myeloma cells, and L929 fibroblast cells. However, probucol treatment significantly inhibited the viability of H929 and RPMI8226 multiple myeloma cells at the concentration of 0.5-10 µM and 5-10 µM, respectively. Conclusion: Probucol treatment slightly inhibited the viability of other cancer cell lines, but significantly inhibited the viability of H929 and RPMI8226 multiple myeloma cells. However, its effect was not potent, since a 50% reduction in cell viability could not be achieved at the concentrations of probucol treatment administered.
Authors and Affiliations
Aslı KOÇ, Arzu Zeynep KARABAY, Ali YAPRAK, Zeliha BÜYÜKBİNGÖL, Fügen AKTAN
Keywords
Related Articles
- EP ID EP693623
- DOI 10.4274/tjps.galenos.2019.04657
- Views 104
- Downloads 0
Determination of the Physicochemical Properties of Piroxicam
Objectives: The aim of this study was to determine the acid dissociation constant (pKa) of piroxicam using high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectrophotometry, and to determin...
Local Use of Apisan Gel, A New Oral Care Product in the Treatment of Experimental Periodontitis Against the Background of Hyperacid Gastritis and Intoxication with Tobacco Smoke
Objectives: The aim of the work was to substantiate the use of a newly created oral care product in the treatment of periodontal disease reconstructed against the background of hyperacidic gastritis under the conditions...
The Cardiopulmonary Effects of the Calcitonin Generelated Peptide Family
Cardiopulmonary diseases are very common among the population. They are high-cost diseases and there are still no definitive treatments. The roles of members of the calcitonin-gene related-peptide (CGRP) family in treati...
Microemulsion Based Gel of Sulconazole Nitrate for Topical Application
Objectives: Sulconazole is a broad spectrum antifungal agent of the imidazole class used against dermatophytes and other fungi to treat skin infections. The aim of the present work was to formulate and evaluate a microem...
Quantitative Structure–Activity Relationship Analysis of Selective Rho Kinase Inhibitors as Neuro-regenerator Agents
Objectives: To understand the role of Rho (serine/threonine) kinases in the treatment of neurological segments, attempts have been made to find potent inhibitors of Rho enzyme by a 2D quantitative structure–activity rela...