DEVELOPMENT OF A NEW CHROMATOGRAPHIC METHOD FOR ESTIMATION OF DOLASETRON IN BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 12
Abstract
Background: A simple, accurate and precise HPLC method for simultaneous determination of Dolasetron in pure and tablet dosage form has been developed. Aim: Development Of A New Chromatographic Method For Estimation Of Dolasetron In Bulk And Pharmaceutical Dosage Form By Rp-Hplc. Materials and Methods: HPLC of Waters (Model: Alliance 2695) with Phenomenex Luna C18 (4.6 mm I.D. × 250 mm, 5 μm) column was used for chromatographic separation. It contains waters injector and PDA Detector (Deuterium). Mobile phase consists of Acetonitrile: Water (50:50% v/v)) and flow rate adjusted was 1ml/min. Wavelength selected for detection was 285nm and injection volume was 10 μl. Results and discussion: By using the developed method, retention time of Dolasetron was found to be 3.008 respectively. The method has been validated for linearity, accuracy and precision. Linearity of Dolasetron were in the range 10– 50μg/ml respectively. The percentage recoveries obtained for Dolasetron were found to be in range of 99.6 LOD and LOQ were found to be 1.16μg/ml and 3.5μg/ml for Dolasetron. Conclusion: A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Dolasetron, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 250mm, 5μm) column using a mixture of Acetonitrile and Water (50:50% v/v) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 285nm. The retention time of the Dolasetron was 3.0 ±0.02min. The method produce linear responses in the concentration range of 10-50ppm of Dolasetron. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Authors and Affiliations
B. Raju
Keywords
Related Articles
- EP ID EP674738
- DOI -
- Views 210
- Downloads 0
HELIOTROPIUM UNDULATUM ATTENUATES HYDRAZINE INDUCED HEPATOTOXICITY: INVOLVEMENT OF CYTOCHROME P450 CYP2E1 AND OXIDATIVE STRESS
Background: Hepatoprotective effect of numerous phytochemicals derived herbal origin have been reported to exhibit chemopreventive potential. The aim of the present study was to evaluate the protective role of Algerian s...
ANTIGLYCATION AND ANTIOXIDANT ACTIVITY OF A HOMEOPATHIC TINCTURE USED IN SRI LANKA
Glycation is the non-enzymatic reaction between a protein and sugars. After a series of complex reactions following glycation, advanced glycation end products (AGEs) are formed. AGEs are responsible for the pathogenesis...
GENERAL REVIEW ON WATERPIPE (NARGHILE)
Waterpipe (hookah, narghile, Shisha) tobacco smoking (WTS) is becoming prevalent worldwide and is one of the most popular forms of tobacco use among youth. WTS prevalence has increased dramatically among young adults and...
OUTCOME OF CLOSE LATERAL INTERNAL ANAL SPHINCTEROTOMY FOR CHRONIC ANAL FISSURE UNDER LOCAL ANESTHESIA
Introduction: Anal fissure is a linear tear in the lower anoderm that presents with moderate to severe pain during and after defecation along with streaks of fresh blood. The pathophysiology is thought to be related to t...
FORCED DEGRADATION AND STABILITY INDICATING STUDIES OF IMATINIB TABLET
Forced degradation or accelerated degradation is a process where drug products are of subjected to excessive chemical and environmental conditions. This study is usually used to identify the possible chemical reactions a...