Development of a PCR-based marker for studying allelic variation of Hd3a in rice and its effect on flowering time
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 4, Issue 5
Abstract
Time of flowering is a key factor affecting both quantity and quality of rice grain production. Among several genes, Hd3a is an important factor associated with flowering time in rice. An Hd3aspecific marker was developed and utilized in a panel of 44 rice indica cultivars with different flowering times to evaluate allelic frequencies and their effect on the trait. Results showed that newly developed marker produced two allelic forms A and B as expected, which had frequencies of 84.1 and 15.9 percent, respectively. Difference between flowering time of cultivars with alleles A and B was significantly large (9.6 days). A relatively high correlation (r=0.408) was detected between flowering time and Hd3a gene. Interval mapping (IM) analysis showed that Hd3a had a positive additive effect (4.8 days) on flowering time and the gene explained 16.2 percent of phenotypic variation of the trait. Our results indicated the applicability of Hd3a-specific marker in studying allelic variation and confirmed previous reports on association of Hd3a with flowering time in rice.
Authors and Affiliations
Leila Nayyeripasand| M.Sc. Student of Agricultural Biotechnology, Department of Agronomy and Plant Breeding. Sari Agricultural Sciences and Natural Resources University, Sari, Iran, Nadali Babaeian Jelodar| Department of Agronomy and Plant Breeding. Sari Agricultural Sciences and Natural Resources University, Sari, Iran, GhorbanAli Nematzadeh| Department of Agronomy and Plant Breeding. Sari Agricultural Sciences and Natural Resources University, Sari, Iran, Asadollah Ahmadikhah*| Department of Agronomy and Plant Breeding. Zanjan University, Zanjan, Iran.ahmadikhaha@gmail.com, Mohammadreza Azimi| Department of Agronomy and Plant Breeding. Zanjan University, Zanjan, Iran.
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