DEVELOPMENT OF AMPEROMETRIC BIOSENSOR FOR GLUTAMATE DETERMINATION
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2015, Vol 12, Issue 1
Abstract
In this work an amperometric biosensor for glutamate determination was developed. Bioselective element of the biosensor was based on enzyme glutamate oxidase, which was immobilized by a covalent cross-linking via glutaraldehyde with bovine serum albumin. Platinum disk electrodes were used as amperometric transducers. The obtained biosensors demonstrated good sensitivity to glutamate. Linear range of glutamate determination was from 2 μM to 600 μM. Influence of properties of working buffer (ionic strength, pH, buffer capacity) on the biosensor work was investigated. It was shown that the developed biosensor is characterized by a good reproducibility of responses during several hours of continuous work and operational stability during several days. The biosensor can be further used for analysis of glutamate concentration in biological or food samples.
Authors and Affiliations
D. Yu. Kucherenko, I. S. Kucherenko, O. O. Soldatkin, T. O. Borisova, S. V. Dzyadevych, A. P. Soldatkin
Keywords
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- EP ID EP399068
- DOI 10.18524/1815-7459.2015.1.104933
- Views 91
- Downloads 0
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