Development of bioprocess for high density cultivation yield of the probiotic [i]Bacillus coagulans[/i] and its spores
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 2
Abstract
[i]Bacillus coagulans[/i] is a spore forming lactic acid bacterium. Spore forming bacteria, have been extensively studied and commercialized as probiotics. Probiotics are produced by fermentation technology. There is a limitation to biomass produced by conventional modes of fermentation. With the great demand generated by range of probiotic products, biomass is becoming very valuable for several pharmaceutical, dairy and probiotic companies. Thus, there is a need to develop high cell density cultivation processes for enhanced biomass accumulation. The bioprocess development was carried out in 6.6 L bench top lab scale fermentor. Four different cultivation strategies were employed to develop a bioprocess for higher growth and sporulation efficiencies of probiotic [i]B. coagulans[/i]. Batch fermentation of [i]B. coagulans [/i]yielded 18 g L[sup]-1[/sup] biomass (as against 8.0 g L[sup]-1[/sup] productivity in shake flask) with 60% spore efficiency. Fed-batch cultivation was carried out for glucose, which yielded 25 g L[sup]-1 [/sup]of biomass. C/N ratio was very crucial in achieving higher spore titres. Maximum biomass yield recorded was 30 g L[sup]-1[/sup], corresponding to 3.8 × 1011 cells mL[sup]-1[/sup] with 81% of cells in sporulated stage. The yield represents increment of 85 times the productivity and 158 times the spore titres relative to the highest reported values for high density cultivation of [i]B. coagulans[/i].
Authors and Affiliations
Kavita Pandey, Babu Vakil
Keywords
Related Articles
- EP ID EP90939
- DOI -
- Views 71
- Downloads 0
A Contribution to the study of ground beetles (Coleoptera: Carabidae) in the Western Rhodope Mts. (Bulgaria)
The available information on the diversity of the ground beetle fauna in the region of the Western Rhodope Mts. was enriched. A total of 133 species were found, including 16 endemic (12% of all) and 5 relict species. Ten...
Rhizosphere bacterial diversity and heavy metal accumulation in Nymphaea pubescens in aid of phytoremediation potential
The present work aims to characterize the bacterial diversity of the rhizosphere system of Nymphaea pubescens and the sediment system where it grows naturally. Heavy metal content in the sediment and Nymphea plant from t...
Phytochemical analysis and antimicrobial activity of baobab (Adansonia digitata) leaves and steam bark extracts on Staphylococcus aureus and Escherichia coli
The phytochemical analysis and antibacterial activity of methanolic and ethanolic leaf and stem bark extracts of baobab tree on Escherichia coli and Staphylococcus aureus were carried out using agar well diffusion method...
Effect of natural bioflavonoid on in vitro ruminal microbiota activity in sheep rumen liquor
A pure bioflavonoid (rutin) was extracted from Eucalyptus globulus leaves and identified by Thin Layer Chromatography using purified flavonoids moieties as a control. The purified flavonoid was used in in vitro gas produ...
Optimization of Neutral Comet Assay for studying DNA double-strand breaks in pea and wheat
This study describes an adaptation of the Comet assay under neutral conditions for mono- and dicotyledonous plants pea (Pisum sativum L.) and wheat (Triticum aestivum L.). Modifications concern lysis and electrophoresis...