Molecular detection of beneficial hyphal soilborn microorganisms in different soil types in areas infested with parasitic broomrapes - Orobanche cumana Wallr. and Phelipanche ramosa L. in Bulgaria
Journal Title: Journal of BioScience and Biotechnology - Year 2018, Vol 7, Issue 2
Abstract
Molecular markers and classic microbiological approaches for assessment of the population density and biodiversity of beneficial hyphal soil-born microorganisms (species from order Actinomicetalеs and arbuscular mycorrhizal fungi from Glomeromycota). Seven different soil types infested by parasitic broomrapes – Orobanche cumana Wallr. and Phelipanche ramosa L. were studied. The soil samples were collected from seventeen regions in Northern and Southern Bulgaria. Soil types were defined as: Chromic Cambisols; Chromic Luvisols; Calcic Vertisols; Mollic Fluvisols; Rendzic Leptosols; Chernozems – Calcic and Haplic, based on agrochemical characteristics. We found that the Chromic Luvisols soils have the highest population density of beneficial microorganisms and a high degree of colonization with arbuscular mycorrhizal fungi, followed by Mollic Fluvisols soils. Two main species of arbuscular mycorrhizal fungi were identified by molecular markers: Glomus intraradices and G. mosseae. The highest density of beneficial streptomycetes was found Chromic Luvisols soils. Based on molecular markers five species of Streptomycetes were identified namely: Streptomyces ambofaciens, S. aureocirculatus, S. carnosus, S. fasiculatus, S. griseorubens. The number of other hyphal fungi was also recorded from genera Aspergillus, Penicillium, Trichoderma, Fusarium, Mucor. Molecular identification of the strains of those fungi, however, will require a metagenomics approach. The results obtained will allow us to develop a new strategy for using beneficial hyphal microorganisms to control parasitic weeds from Orobanchaceae.
Authors and Affiliations
Ina Kirilova, Tsveta H. Hristeva, Radka Bozhinova, Iliya Denev, Margarita Docheva, Yonko Yonchev
Keywords
Related Articles
- EP ID EP603334
- DOI -
- Views 57
- Downloads 0
Sensitivity of two garden pea genotypes to physical and chemical mutagens
A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sati...
Physiological damage in Algerian mouse Mus spretus (Rodentia: Muridae) exposed to crude oil
Small mammals have been used to predict ecotoxicological damage caused by metals in field studies and laboratory exposure. In natural ecosystems, rodents play an important role either as seed dispersers or food providers...
Meiotic changes in Vicia faba L. subsequent to treatments of hydrazine hydrate and maleic hydrazide
Assessing the impact of mutagens for creating variations in crops like faba bean (Vicia faba L.) is an important criterion in the contemporary world where food insecurity and malnutrition is alarming at the doors of vari...
Effect of different concentrations of aqueous extract of ginger (Zingiber officinale) on performance and carcass characteristics of male broiler chickens in wheat-soybean meal based diets
This study was conducted to investigate the effects of using different concentrations of ginger extract (Zingiber officinale) on performance and carcass characteristics of male broiler chickens. Three hundred one-day-old...
Aberrant promoter methylation in p53 and ATM genes was not associated with sporadic breast carcinogenesis in Bulgarian patients
Epigenetic silencing of gene expression has become an intensively studied topic regarding breast cancer during the last years. Previous investigations have shown that hypermethylation in gene promoters is an altern...