Evaluation of salinity tolerance in maize (Zea mays L.) genotypes at seedling stage
Journal Title: Journal of BioScience and Biotechnology - Year 2015, Vol 4, Issue 1
Abstract
Reproducible and reliable evaluation methods are the basis of any successful breeding programme especially for abiotic breeding. In this study, solutionculture and vermiculite-culture methods were employed to evaluate the salinity tolerance of nine maize genotypes. Ten seedling traits (shoot length, root length, leaf number, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, Na+ content, K+ content and K+ /Na+ ratio) were measured under 0 mM, 100 mM and 200 mM NaCl salinity stress and similar results were observed in both systems. Among the genotypes under investigation CZ-7 expressed as the tolerant and B73 appeared to be more sensitive. Biomass especially shoot fresh weight has shown a major growth reduction compare to the control which would be very useful trait in salinity tolerance improvement programme. The newly developed vermiculite-culture method was found an effective, easy and affordable technique for assessment of salt tolerance and which will be useful for plant breeders.
Authors and Affiliations
MOHAMMAD IBNE HOQUE, ZHENG JUN, WANG GUOYING
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