Exploration on production of rhamnolipid biosurfactants using native Pseudomonas aeruginosa strains
Journal Title: Journal of BioScience and Biotechnology - Year 2015, Vol 4, Issue 2
Abstract
Biosurfactants are structurally diverse surface-active molecules, produced on living surfaces, mostly microbial cell surfaces or excreted extracellularly. Rhamnolipid biosurfactants have wide spectrum use and are predominantly produced by the bacteria Pseudomonas aeruginosa. In this study, 75 Pseudomonas strains isolated from distinct native habitats were screened following oil spreading technique, methylene blue agar method, hemolytic blood agar method and surface tension measurement of the cell free culture. Ten selected isolates were tested for their ability to produce rhamnolipid biosurfactants in glycerol mediated broth. The best among them, Pa24, was confirmed as Pseudomonas aeruginosa through 16S rRNA sequence analysis. Experiments carried out on the ability of P. aeruginosa strain Pa24 revealed its potential to utilize range of vegetable oils such as coconut oil, palm oil, jatropha oil, neem oil and mineral glycerol as sole source of carbon and produce rhamnolipid biosurfactant. The extracted biosurfactant was characterized by thin layer chromatography and high performance thin layer chromatography as mixture of di-rhamnolipid and mono-rhamnolipid biosurfactants. The crude extract of rhamnolipid was tested in-vitro for antifungal activity against Phytophthora capsici and Phytophthora infestans and the MIC50 were found to be 815.8 ppm and 373.9 ppm, respectively. Further exploration on different renewable carbon sources including agriculture industrial wastes to produce rhamnolipid biosurfactants can improve the efficiency and reduce the environmental pollution through waste discharge from these industries.
Authors and Affiliations
RAVISH BHAT, DAYAMANI J. , SHREEKARA HATHWAR, RAJENDRA HEGDE, ANIL KUSH
Keywords
Related Articles
- EP ID EP143042
- DOI -
- Views 365
- Downloads 0
Physiological, nutritional, and biochemical responses under nickel toxicity in black poplar (Populus nigra)
Nickel (Ni) is an essential nutrient for plants and it has been identified as a component of a number of enzymes such as ureases. In this study, we have studied the long-term effects of nickel toxicity on black poplar (P...
Phenolic acid profiles of endemic species Verbascum anisophyllum and Verbascum davidoffii
The profiles of methanol extractable and methanol insoluble bound phenolic acids of two species: Verbascum anisophyllum Murb (Balkan endemic) and Verbascum davidoffii Murb. (Bulgarian endemic) were determined. Free radic...
Occurrence of hydrocarbon-degrading nitrogen-fixing bacteria in the rhizosphere of Paspalum vaginatum Sw.
This study investigated the influence of nitrogen-fixing hydrocarbon-degrading bacteria from the rhizosphere of plants in the biodegradation of crude oil in the polluted soil. Several nitrogen-fixing and crude oil utiliz...
Sensitivity of two garden pea genotypes to physical and chemical mutagens
A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sati...
Investigation of post-fire natural regeneration in forest plantations of Pinus sylvestris and Larix decidua on the Northern slopes of Rila mountain
Wildfires alter both the vegetation and the soil properties, thus changing the conditions of their regeneration. Each year, forest fires impact significant areas within the lower forest zone, where the coniferous plantat...