Sensitivity of two garden pea genotypes to physical and chemical mutagens
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 2
Abstract
A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sativum L. genotypes to physical and chemical mutagens. In the experiment, the pea seeds were irradiated single or combined with 60Co gamma rays (40, 80, 100, 200 and 400 Gy) and Ethyl methanesulfonate (EMS) at concentrations of 0.1 and 0.2 %. Visible morphological changes of the stems and leaves were observed in plant of M1 generation, such as shorter stems, double petiole, clover-shaped leaves and a couple of tendrils. Single treatments with 100 Gy 60Co induced the highest mutation frequency M.F. = 7.69 for line 88-7, and М.F. = 2.11 for line 97-3, reported in M2 generation. Higher doses or combined gamma rays and EMS treatments induced more efficiently mutations. Line 88-7 was selected for further mutagenic treatment due to the higher sensitivity assessed.
Authors and Affiliations
Slavka Kalapchieva , Nasya Tomlekova
Keywords
Related Articles
- EP ID EP90938
- DOI -
- Views 99
- Downloads 0
Immunohistochemical study of IL-17, perforin and CD68 in colorectal cancer
The tumor development in colorectal cancer is one of the most investigated tumorigenesis in the world. This study aims to analyze the impact of presence of IL-17, perforin and CD68 on colorectal cancer. During the...
Effects of carbon sources, oxygenation and ethanol on the production of inulinase by Kluyveromyces marxianus YX01
Inulinase is one of the most important factors in consolidated bioprocessing, which combines enzyme production, inulin saccharification, and ethanol fermentation into a single process. In our study, inulinase production...
New data on zooplankton communities of two Bulgarian reservoirs: Konush and Sinyata Reka, Plovdiv Region
Zooplankton communities have several vital functions within lake ecosystems, including the transfer of energy and material from producers to secondary consumers. Their structure is potentially affected by both lake morph...
Optimized production of extracellular proteases by [i]Bacillus subtilis[/i] from degraded abattoir waste
Proteases are ubiquitous in occurrence and are found in all living organisms. These are essential for cell growth and differentiation. The extracellular proteases are of a high commercial value and find multiple applicat...
The effect of saline water on quantitative and qulaitive charactristics of melon genotypes
Currently, the development of salt tolerant cultivars is one of the most effective methods to increase the efficiency of saline soils and water resources in arid and semi-arid regions of the world. In this regard, the ef...