Sensitivity of two garden pea genotypes to physical and chemical mutagens
Journal Title: Journal of BioScience and Biotechnology - Year 2016, Vol 5, Issue 2
Abstract
A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sativum L. genotypes to physical and chemical mutagens. In the experiment, the pea seeds were irradiated single or combined with 60Co gamma rays (40, 80, 100, 200 and 400 Gy) and Ethyl methanesulfonate (EMS) at concentrations of 0.1 and 0.2 %. Visible morphological changes of the stems and leaves were observed in plant of M1 generation, such as shorter stems, double petiole, clover-shaped leaves and a couple of tendrils. Single treatments with 100 Gy 60Co induced the highest mutation frequency M.F. = 7.69 for line 88-7, and М.F. = 2.11 for line 97-3, reported in M2 generation. Higher doses or combined gamma rays and EMS treatments induced more efficiently mutations. Line 88-7 was selected for further mutagenic treatment due to the higher sensitivity assessed.
Authors and Affiliations
Slavka Kalapchieva , Nasya Tomlekova
Keywords
Related Articles
- EP ID EP90938
- DOI -
- Views 120
- Downloads 0
Molecular detection of beneficial hyphal soilborn microorganisms in different soil types in areas infested with parasitic broomrapes - Orobanche cumana Wallr. and Phelipanche ramosa L. in Bulgaria
Molecular markers and classic microbiological approaches for assessment of the population density and biodiversity of beneficial hyphal soil-born microorganisms (species from order Actinomicetalеs and arbuscular mycorrhi...
Ethnobotanical study of medicinal plants of Mirab-Badwacho district, Ethiopia
Analyzing inhibition of BCL11A gene expression in K562 cells by RNAi
RNA interference (RNAi), an effective approach to sequence-specific gene knockdown is widely used for the investigation of regulation of gene expression in various cells. BCL11A (B cell lymphoma 11A) plays a vital role i...
Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells
Medicinal plants have been intensively studied as a source of antitumor compounds. Due to the beneficial climate conditions Bulgarian herbs have high pharmacological potential. Currently, the antitumor effect of the Bulg...
Optimization of nutritive medium composition for production of amylase by Bacillus strains
The current study aimed to examine the amylolytic activities of various species of the Bacillus genus. A total of 31% of the tested 166 strains showed a positive reaction on starch agar. Their amylolytic activity was in...